Bilirubin determination at the electrified liquid-liquid interface supported with a 3D printed capillary

0574195 - ÚFCH JH 2024 RIV NL eng J - Journal Article
Sobczak, K. - Rudnicki, K. - Bobrowska, D. M. - Kolivoška, Viliam - Poltorak, L.
Bilirubin determination at the electrified liquid-liquid interface supported with a 3D printed capillary.
Sensors and Actuators B - Chemical. Roč. 394, NOV 2023 (2023), č. článku 134360. E-ISSN 0925-4005
Institutional support: RVO:61388955
Keywords : Fused deposition modeling * ITIES * voltammetry
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Impact factor: 8.4, year: 2022
Method of publishing: Open access

In this work, we have studied the behavior of bilirubin at the electrified liquid-liquid interface using two voltammetric techniques - cyclic and differential pulse voltammetry. The effect of bilirubin concentration as the analyte, and the influence of the aqueous phase pH on the interfacial behavior were investigated. Our focus was to optimize the electroanalytical determination protocol of bilirubin at the electrified liquid-liquid interface with the buffered aqueous phase (physicochemical study) and/or artificial urine formulation (analytical study). In this respect, we have extracted a number of electroanalytical parameters concerning bilirubin covering limits of detection and quantification and sensitivities. To push the optimized protocol towards applicability, we have designed and fabricated a 3D printed polyamide based tube finished with an aperture supporting a soft junction. Liquid-liquid interface placed in a fabricated support was characterized using voltammetry and scanning electron microscopy. With 3D printed platform we have significantly reduced the amount of consumed chemicals (from a few mL to a few tens of µL) and obtained a few times lower limit of determination as compared with the macroscopic apparatus.
Permanent Link: https://hdl.handle.net/11104/0344543