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Lab-on-chip microscope platform for electro-manipulation of a dense microtubules network
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SYSNO ASEP 0567065 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Lab-on-chip microscope platform for electro-manipulation of a dense microtubules network Author(s) Havelka, Daniel (URE-Y) RID
Zhernov, I. (CZ)
Teplan, M. (SK)
Lánský, Z. (CZ)
Chafai, Djamel Eddine (URE-Y)
Cifra, Michal (URE-Y) RID, ORCID, SAINumber of authors 6 Article number 2462 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 12, č. 1 (2022)Number of pages 12 s. Publication form Print - P Language eng - English Country DE - Germany Keywords kinesin motors ; tubulin ; migration ; alignment ; cells Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering OECD category Electrical and electronic engineering R&D Projects GA18-23597S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support URE-Y - RVO:67985882 UT WOS 000755212600015 EID SCOPUS 85124615232 DOI 10.1038/s41598-022-06255-y Annotation Pulsed electric field (PEF) technology is promising for the manipulation of biomolecular components and has potential applications in biomedicine and bionanotechnology. Microtubules, nanoscopic tubular structures self-assembled from protein tubulin, serve as important components in basic cellular processes as well as in engineered biomolecular nanosystems. Recent studies in cell-based models have demonstrated that PEF affects the cytoskeleton, including microtubules. However, the direct effects of PEF on microtubules are not clear. In this work, we developed a lab-on-a-chip platform integrated with a total internal reflection fluorescence microscope system to elucidate the PEF effects on a microtubules network mimicking the cell-like density of microtubules. The designed platform enables the delivery of short (microsecond-scale), high-field-strength (<= 25 kV/cm) electric pulses far from the electrode/electrolyte interface. We showed that microsecond PEF is capable of overcoming the non-covalent microtubule bonding force to the substrate and translocating the microtubules. This microsecond PEF effect combined with macromolecular crowding led to aggregation of microtubules. Our results expand the toolbox of bioelectronics technologies and electromagnetic tools for the manipulation of biomolecular nanoscopic systems and contribute to the understanding of microsecond PEF effects on a microtubule cytoskeleton Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2023 Electronic address https://doi.org/10.1038/s41598-022-06255-y
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