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A bacterium from a mountain lake harvests light using both proton-pumping xanthorhodopsins and bacteriochlorophyll-based photosystems
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SYSNO ASEP 0565420 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A bacterium from a mountain lake harvests light using both proton-pumping xanthorhodopsins and bacteriochlorophyll-based photosystems Author(s) Kopejtka, Karel (MBU-M) ORCID, RID
Tomasch, Jurgen (MBU-M) ORCID, RID
Kaftan, David (MBU-M) ORCID
Gardiner, Alastair T. (MBU-M) ORCID
Bína, David (BC-A) RID, ORCID
Gardian, Zdenko (BC-A) RID
Bellas, Ch. (AT)
Dröge, A. (DE)
Geffers, R. (DE)
Sommaruga, R. (DE)
Koblížek, Michal (MBU-M) RID, ORCIDArticle number e2211018119 Source Title Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 119, č. 50 (2022)Number of pages 10 s. Language eng - English Country US - United States Keywords anoxygenic photosynthesis ; bacteriochlorophyll a ; dual phototrophy ; light energy ; xanthorhodopsin Subject RIV EE - Microbiology, Virology OECD category Microbiology Subject RIV - cooperation Biology Centre (since 2006) - Cell Biology R&D Projects GX19-28778X GA ČR - Czech Science Foundation (CSF) EF18_053/0017705 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 ; BC-A - RVO:60077344 UT WOS 000944610300002 EID SCOPUS 85143569135 DOI 10.1073/pnas.2211018119 Annotation Photoheterotrophic bacteria harvest light energy using either proton-pumping rhodopsins or bacteriochlorophyll (BChl)-based photosystems. The bacterium Sphingomonas glacialis AAP5 isolated from the alpine lake Gossenköllesee contains genes for both systems. Here, we show that BChl is expressed between 4°C and 22°C in the dark, whereas xanthorhodopsin is expressed only at temperatures below 16°C and in the presence of light. Thus, cells grown at low temperatures under a natural light-dark cycle contain both BChl-based photosystems and xanthorhodopsins with a nostoxanthin antenna. Flash photolysis measurements proved that both systems are photochemically active. The captured light energy is used for ATP synthesis and stimulates growth. Thus, S. glacialis AAP5 represents a chlorophototrophic and a retinalophototrophic organism. Our analyses suggest that simple xanthorhodopsin may be preferred by the cells under higher light and low temperatures, whereas larger BChl-based photosystems may perform better at lower light intensities. This indicates that the use of two systems for light harvesting may represent an evolutionary adaptation to the specific environmental conditions found in alpine lakes and other analogous ecosystems, allowing bacteria to alternate their light-harvesting machinery in response to large seasonal changes of irradiance and temperature. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://www.pnas.org/doi/10.1073/pnas.2211018119
Number of the records: 1