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Cathodoluminescence imaging of cellular structures labeled with luminescent iridium or rhenium complexes at cryogenic temperatures
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SYSNO ASEP 0565268 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cathodoluminescence imaging of cellular structures labeled with luminescent iridium or rhenium complexes at cryogenic temperatures Author(s) Vancová, Marie (BC-A) RID, ORCID
Skoupý, Radim (UPT-D) RID, ORCID, SAI
Ďurinová, Eva (BC-A) ORCID
Bílý, Tomáš (BC-A) ORCID
Nebesářová, Jana (BC-A) RID, ORCID
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Kolouch, A. (CZ)
Horodysky, P. (CZ)Number of authors 8 Article number 13432 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 12, č. 1 (2022)Number of pages 9 s. Publication form Online - E Language eng - English Country DE - Germany Keywords Cathodoluminescence ; cellular ; cryogenic Subject RIV EA - Cell Biology OECD category Cell biology R&D Projects LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) TN01000008 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Method of publishing Open access Institutional support BC-A - RVO:60077344 ; UPT-D - RVO:68081731 UT WOS 000836406600047 EID SCOPUS 85135396685 DOI 10.1038/s41598-022-17723-w Annotation We report for the first time the use of two live-cell imaging agents from the group of luminescent transition metal complexes (IRAZOLVE-MITO and REZOLVE-ER) as cathodoluminescent probes. This first experimental demonstration shows the application of both probes for the identification of cellular structures at the nanoscale and near the native state directly in the cryo-scanning electron microscope. This approach can potentially be applied to correlative and multimodal approaches and used to target specific regions within vitrified samples at low electron beam energies. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2023 Electronic address https://www.nature.com/articles/s41598-022-17723-w
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