Genome sequence and silkomics of the spindle ermine moth, Yponomeuta cagnagella, representing the early diverging lineage of the ditrysian Lepidoptera

Voleníková, Anna; Nguyen, Petr; Davey, P.; Sehadová, Hana; Kludkiewicz, Barbara; Koutecký, P.; Walters, J. R.; Roessingh, P.; Provazníková, Irena; Šerý, Michal; Žurovcová, Martina; Hradilová, Miluše; Rouhová, Lenka; Žurovec, Michal

doi:https://dx.doi.org/10.1038/s42003-022-04240-9

Number of the records: 1

Genome sequence and silkomics of the spindle ermine moth, Yponomeuta cagnagella, representing the early diverging lineage of the ditrysian Lepidoptera

1.

SYSNO ASEP	0564417
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Genome sequence and silkomics of the spindle ermine moth, Yponomeuta cagnagella, representing the early diverging lineage of the ditrysian Lepidoptera
Author(s)	Voleníková, Anna (BC-A)_ORCID Nguyen, Petr (BC-A)_{RID, ORCID} Davey, P. (GB) Sehadová, Hana (BC-A)_{RID, ORCID} Kludkiewicz, Barbara (BC-A)_ORCID Koutecký, P. (CZ) Walters, J. R. (US) Roessingh, P. (NL) Provazníková, Irena (BC-A)_ORCID Šerý, Michal (BC-A)_ORCID Žurovcová, Martina (BC-A)_{RID, ORCID} Hradilová, Miluše (UMG-J) Rouhová, Lenka (BC-A)_ORCID Žurovec, Michal (BC-A)_{RID, ORCID}
Number of authors	14
Article number	1281
Source Title	Communications Biology. - : Nature Publishing Group Roč. 5, č. 1 (2022)
Number of pages	13 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	linked oligosaccharide chains ; silk fibroin ; Bombyx mori
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Developmental biology
Subject RIV - cooperation	Institute of Molecular Genetics - Genetics ; Molecular Biology
R&D Projects	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	BC-A - RVO:60077344 ; UMG-J - RVO:68378050
UT WOS	000887050100001
EID SCOPUS	85137223171
DOI	10.1038/s42003-022-04240-9
Annotation	Many lepidopteran species produce silk, cocoons, feeding tubes, or nests for protection from predators and parasites for caterpillars and pupae. Yet, the number of lepidopteran species whose silk composition has been studied in detail is very small, because the genes encoding the major structural silk proteins tend to be large and repetitive, making their assembly and sequence analysis difficult. Here we have analyzed the silk of Yponomeuta cagnagella, which represents one of the early diverging lineages of the ditrysian Lepidoptera thus improving the coverage of the order. To obtain a comprehensive list of the Y. cagnagella silk genes, we sequenced and assembled a draft genome using Oxford Nanopore and Illumina technologies. We used a silk-gland transcriptome and a silk proteome to identify major silk components and verified the tissue specificity of expression of individual genes. A detailed annotation of the major genes and their putative products, including their complete sequences and exonintron structures is provided. The morphology of silk glands and fibers are also shown. This study fills an important gap in our growing understanding of the structure, evolution, and function of silk genes and provides genomic resources for future studies of the chemical ecology of Yponomeuta species.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2023
Electronic address	https://www.nature.com/articles/s42003-022-04240-9.pdf

Number of the records: 1