Brassinosteroids application induces phosphatidic acid production and modify antioxidant enzymes activity in tobacco in calcium-dependent manner

0545886 - ÚEB 2022 RIV US eng J - Journal Article
Kretynin, S. V. - Kolesnikov, Y. S. - Derevyanchuk, M. - Kalachova, Tetiana - Blume, Y. B. - Khripach, V.A. - Kravets, V. S.
Brassinosteroids application induces phosphatidic acid production and modify antioxidant enzymes activity in tobacco in calcium-dependent manner.
Steroids. Roč. 168, APR (2021), č. článku 108444. ISSN 0039-128X. E-ISSN 1878-5867
R&D Projects: GA MŠMT(CZ) EF16_019/0000738
Institutional support: RVO:61389030
Keywords : Antioxidant enzymes * Brassinosteroids * Calcium * Nicotiana tabacum * Phosphatidic acid
OECD category: Biochemical research methods
Impact factor: 2.760, year: 2021
Method of publishing: Open access
http://doi.org/10.1016/j.steroids.2019.108444

Brassinosteroids (BRs) are steroid hormones regulating various aspects of plant metabolism, including growth, development and stress responses. However, little is known about the mechanism of their impact on antioxidant systems and phospholipid turnover. Using tobacco plants overexpressing H+/Ca2+vacuolar Arabidopsis antiporter CAX1, we showed the role of Ca2+ ion balance in the reactive oxygen species production and rapid phosphatidic acid accumulation induced by exogenous BR. Combination of our experimental results with public transcriptomic and proteomic data revealed a particular role of Ca2+-dependent phospholipid metabolizing enzymes in BR signaling. Here we provide novel insights into the role of calcium balance and lipid-derived second messengers in plant responses to exogenous BRs and propose a complex model integrating BR-mediated metabolic changes with phospholipid turnover.
Permanent Link: http://hdl.handle.net/11104/0322512