Number of the records: 1
Transcriptome sequencing and multi-plex imaging of prostate cancer microenvironment reveals a dominant role for monocytic cells in progression
- 1.
SYSNO ASEP 0544993 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transcriptome sequencing and multi-plex imaging of prostate cancer microenvironment reveals a dominant role for monocytic cells in progression Author(s) Mangiola, S. (AU)
McCoy, P. (AU)
Modrák, Martin (MBU-M) ORCID
Souza-Fonseca-Guimaraes, F. (AU)
Blashki, D. (AU)
Stuchbery, R. (AU)
Keam, S. P. (AU)
Kerger, M. (AU)
Chow, K. (AU)
Nasa, C. (AU)
Le Page, M. (AU)
Lister, N. (AU)
Monard, S. (AU)
Peters, J. (AU)
Dundee, P. (AU)
Williams, S. G. (AU)
Costello, A. J. (AU)
Neeson, P. J. (AU)
Pal, B. (AU)
Huntington, N. D. (AU)
Corcoran, N. M. (AU)
Papenfuss, A. T. (AU)
Hovens, C. M. (AU)Article number 846 Source Title Bmc Cancer. - : BioMed Central
Roč. 21, č. 1 (2021)Number of pages 18 s. Language eng - English Country GB - United Kingdom Keywords Prostate cancer ; Transcriptomics ; facs ; Immunohistochemistry ; Deconvolution ; Bayes ; Differential gene expression ; capra-s ; Microenvironment ; Epithelial ; Myeloid ; Macrophages ; Cholesterol ; pdl1 Subject RIV FD - Oncology ; Hematology OECD category Oncology R&D Projects LM2015047 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000680321500003 EID SCOPUS 85111046075 DOI 10.1186/s12885-021-08529-6 Annotation Background Prostate cancer is caused by genomic aberrations in normal epithelial cells, however clinical translation of findings from analyses of cancer cells alone has been very limited. A deeper understanding of the tumour microenvironment is needed to identify the key drivers of disease progression and reveal novel therapeutic opportunities. Results In this study, the experimental enrichment of selected cell-types, the development of a Bayesian inference model for continuous differential transcript abundance, and multiplex immunohistochemistry permitted us to define the transcriptional landscape of the prostate cancer microenvironment along the disease progression axis. An important role of monocytes and macrophages in prostate cancer progression and disease recurrence was uncovered, supported by both transcriptional landscape findings and by differential tissue composition analyses. These findings were corroborated and validated by spatial analyses at the single-cell level using multiplex immunohistochemistry. Conclusions This study advances our knowledge concerning the role of monocyte-derived recruitment in primary prostate cancer, and supports their key role in disease progression, patient survival and prostate microenvironment immune modulation. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://bmccancer.biomedcentral.com/articles/10.1186/s12885-021-08529-6
Number of the records: 1