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Influence of Lipid Membranes on α-Synuclein Aggregation
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SYSNO ASEP 0541374 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Influence of Lipid Membranes on α-Synuclein Aggregation Author(s) Kurochka, Andrii (UOCHB-X) ORCID
Yushchenko, Dmytro A. (UOCHB-X) ORCID, RID
Bouř, Petr (UOCHB-X) RID, ORCID
Shvadchak, Volodymyr V. (UOCHB-X) ORCID, RIDSource Title ACS Chemical Neuroscience. - : American Chemical Society - ISSN 1948-7193
Roč. 12, č. 5 (2021), s. 825-830Number of pages 6 s. Language eng - English Country US - United States Keywords amyloid ; fibril ; fibrillization ; kinetics ; lag time ; liposomes OECD category Biophysics R&D Projects GJ18-06255Y GA ČR - Czech Science Foundation (CSF) GA20-10144S GA ČR - Czech Science Foundation (CSF) EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UOCHB-X - RVO:61388963 UT WOS 000626321800002 EID SCOPUS 85101666075 DOI 10.1021/acschemneuro.0c00819 Annotation α-Synuclein is a neuronal protein involved in synaptic vesicle trafficking. During the course of Parkinson's disease, it aggregates, forming amyloid fibrils that accumulate in the midbrain. This pathological fibrillization process is strongly modulated by physiological interactions of α-synuclein with lipid membranes. However, the detailed mechanism of this effect remains unclear. In this work, we used environment-sensitive fluorescent dyes to study the influence of model lipid membranes on the kinetics of α-synuclein fibrillization. We observed that formation of the fibrils from α-synuclein monomers is strongly delayed even by small amounts of lipids. Furthermore, we found that membrane-bound α-synuclein monomers are not involved in fibril elongation. Hence, presence of lipids slows down fibril growth proportionally to the fraction of membrane-bound protein. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2022 Electronic address https://doi.org/10.1021/acschemneuro.0c00819
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