Number of the records: 1
16S rRNA Gene Copy Number Normalization Does Not Provide More Reliable Conclusions in Metataxonomic Surveys
- 1.
SYSNO ASEP 0541306 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title 16S rRNA Gene Copy Number Normalization Does Not Provide More Reliable Conclusions in Metataxonomic Surveys Author(s) Starke, Robert (MBU-M) ORCID, RID
Pylro, V. S. (BR)
Morais, Daniel (MBU-M) ORCIDSource Title Microbial Ecology. - : Springer - ISSN 0095-3628
Roč. 81, č. 2 (2021), s. 535-539Number of pages 5 s. Language eng - English Country US - United States Keywords 16S rRNA ; Gene ; Metataxonomic surveys Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA18-25706S GA ČR - Czech Science Foundation (CSF) GJ20-02022Y GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000563713400001 EID SCOPUS 85089975354 DOI 10.1007/s00248-020-01586-7 Annotation Sequencing 16S rRNA gene amplicons is the gold standard to uncover the composition of prokaryotic communities. The presence of multiple copies of this gene makes the community abundance data distorted and gene copy normalization (GCN) necessary for correction. Even though GCN of 16S data provided a picture closer to the metagenome before, it should also be compared with communities of known composition due to the fact that library preparation is prone to methodological biases. Here, we process 16S rRNA gene amplicon data from eleven simple mock communities with DADA2 and estimate the impact of GCN. In all cases, the mock community composition derived from the 16S sequencing differs from those expected, and GCN fails to improve the classification for most of the analysed communities. Our approach provides empirical evidence that GCN does not improve the 16S target sequencing analyses in real scenarios. We therefore question the use of GCN for metataxonomic surveys until a more comprehensive catalogue of copy numbers becomes available. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://link.springer.com/article/10.1007%2Fs00248-020-01586-7
Number of the records: 1