Number of the records: 1
The genotoxic effects in the leukocytes of workers handling nanocomposite materials
- 1.
SYSNO ASEP 0534364 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The genotoxic effects in the leukocytes of workers handling nanocomposite materials Author(s) Novotná, Božena (UEM-P)
Pelclová, D. (CZ)
Rössnerová, Andrea (UEM-P) RID
Ždímal, Vladimír (UCHP-M) RID, ORCID, SAI
Ondráček, Jakub (UCHP-M) RID, ORCID, SAI
Lischková, L. (CZ)
Vlčková, Š. (CZ)
Fenclová, Z. (CZ)
Klusáčková, P. (CZ)
Závodná, Táňa (UEM-P)
Topinka, Jan (UEM-P) RID, ORCID
Komarc, M. (CZ)
Dvořáčková, Š. (CZ)
Rössner ml., Pavel (UEM-P) RID, ORCIDSource Title Mutagenesis. - : Oxford University Press - ISSN 0267-8357
Roč. 35, č. 4 (2020), s. 331-340Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords obesity ; exposure ; aerosols Subject RIV EB - Genetics ; Molecular Biology OECD category Occupational health Subject RIV - cooperation Institute of Chemical Process Fundamentals - Health Impact of the Environment Quality R&D Projects GA18-02079S GA ČR - Czech Science Foundation (CSF) LO1508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018124 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UEM-P - RVO:68378041 ; UCHP-M - RVO:67985858 UT WOS 000606985200004 EID SCOPUS 85083093582 DOI 10.1093/mutage/geaa016 Annotation The extensive development of nanotechnologies and nanomaterials poses a number of questions to toxicologists about the potential health risks of exposure to nanoparticles (NP). In this study, we analysed DNA damage in the leukocytes of 20 workers who were long-term exposed (18 ± 10 years) to NP in their working environment. Blood samples were collected in September 2016, before and after a shift, to assess (i) the chronic effects of NP on DNA (pre-shift samples) and (ii) the acute effects of exposure during the shift (the difference between pre- and post-shift samples). The samples from matched controls were taken in parallel with workers before the shift. Leukocytes were isolated from heparinised blood on a Ficoll gradient. The enzyme-modified comet assay (DNA formamido-pyrimidine-glycosylase and endonuclease III) demonstrated a considerable increase of both single- and double-strand breaks in DNA (DNA-SB) and oxidised bases when compared with the controls (2.4× and 2×, respectively). Acute exposure induced a further increase of DNA-SB. The welding and smelting of nanocomposites represented a higher genotoxic risk than milling and grinding of nanocomposite surfaces. Obesity appeared to be a factor contributing to an increased risk of oxidative damage to DNA. The data also indicated a higher susceptibility of males vs. females to NP exposure. The study was repeated in September 2017. The results exhibited similar trend, but the levels of DNA damage in the exposed subjects were lower compared to previous year. This was probably associated with lower exposure to NP in consequence of changes in nanomaterial composition and working operations. The further study involving also monitoring of personal exposures to NP is necessary to identify (i) the main aerosol components responsible for genotoxic effects in workers handling nanocomposites and (ii) the primary cause of gender differences in response to NP action Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2021 Electronic address https://academic.oup.com/mutage/article-abstract/35/4/331/5875532?redirectedFrom=fulltext
Number of the records: 1