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Extraordinary Sequence Diversity and Promiscuity of Centromeric Satellites in the Legume Tribe Fabeae.
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SYSNO ASEP 0532455 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Extraordinary Sequence Diversity and Promiscuity of Centromeric Satellites in the Legume Tribe Fabeae. Author(s) Robledillo, Laura Avila (BC-A) RID
Neumann, Pavel (BC-A) RID, ORCID
Koblížková, Andrea (BC-A)
Novák, Petr (BC-A) RID, ORCID
Vrbová, Iva (BC-A) RID, ORCID
Macas, Jiří (BC-A) RID, ORCID, SAINumber of authors 6 Source Title Molecular Biology and Evolution. - : Oxford University Press - ISSN 0737-4038
Roč. 37, č. 8 (2020), s. 2341-2356Number of pages 16 s. Language eng - English Country US - United States Keywords centromere evolution ; satellite DNA ; CENH3 ; ChIP-seq ; plant chromosomes Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects GA17-09750S GA ČR - Czech Science Foundation (CSF) LM2015047 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000574381000016 EID SCOPUS 85089203169 DOI 10.1093/molbev/msaa090 Annotation Satellite repeats are major sequence constituents of centromeres in many plant and animal species. Within a species, a single family of satellite sequences typically occupies centromeres of all chromosomes and is absent from other parts of the genome. Due to their common origin, sequence similarities exist among the centromere-specific satellites in related species. Here, we report a remarkably different pattern of centromere evolution in the plant tribe Fabeae, which includes genera Pisum, Lathyrus, Vicia, and Lens. By immunoprecipitation of centromeric chromatin with CENH3 antibodies, we identified and characterized a large and diverse set of 64 families of centromeric satellites in 14 species. These families differed in their nucleotide sequence, monomer length (33-2,979bp), and abundance in individual species. Most families were species-specific, and most species possessed multiple (2-12) satellites in their centromeres. Some of the repeats that were shared by several species exhibited promiscuous patterns of centromere association, being located within CENH3 chromatin in some species, but apart from the centromeres in others. Moreover, FISH experiments revealed that the same family could assume centromeric and noncentromeric positions even within a single species. Taken together, these findings suggest that Fabeae centromeres are not shaped by the coevolution of a single centromeric satellite with its interacting CENH3 proteins, as proposed by the centromere drive model. This conclusion is also supported by the absence of pervasive adaptive evolution of CENH3 sequences retrieved from Fabeae species. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2021 Electronic address https://academic.oup.com/mbe/article/37/8/2341/5817320
Number of the records: 1