Analysis of HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas and Paired Normal Mucosae Reveals Cyclin D1 Deregulation and Compensatory Effect of Cyclin D2

Novotný, Jiří; Bandurova, V.; Strnad, Hynek; Chovanec, M.; Hradilová, Miluše; Šáchová, Jana; Steffl, M.; Grušanovič, Josipa; Kodet, R.; Pačes, Václav; Lacina, L.; Smetana, K. , Jr.; Plzák, J.; Kolář, Michal; Vomastek, Tomáš

doi:https://dx.doi.org/10.3390/cancers12040792

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Analysis of HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas and Paired Normal Mucosae Reveals Cyclin D1 Deregulation and Compensatory Effect of Cyclin D2

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SYSNO ASEP	0525100
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Analysis of HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas and Paired Normal Mucosae Reveals Cyclin D1 Deregulation and Compensatory Effect of Cyclin D2
Author(s)	Novotný, Jiří (UMG-J)_ORCID Bandurova, V. (CZ) Strnad, Hynek (UMG-J)_RID Chovanec, M. (CZ) Hradilová, Miluše (UMG-J) Šáchová, Jana (UMG-J) Steffl, M. (CZ) Grušanovič, Josipa (MBU-M) Kodet, R. (CZ) Pačes, Václav (UMG-J)_RID Lacina, L. (CZ) Smetana, K. , Jr. (CZ) Plzák, J. (CZ) Kolář, Michal (UMG-J)_{RID, ORCID} Vomastek, Tomáš (MBU-M)_{RID, ORCID}
Number of authors	15
Article number	792
Source Title	Cancers (Basel). - : MDPI Roč. 12, č. 4 (2020)
Number of pages	19 s.
Publication form	Online - E
Language	eng - English
Country	CH - Switzerland
Keywords	human papillomavirus ; head and neck squamous cell carcinoma ; cell cycle ; D-type cyclins ; ccnd1 ; ccnd2 ; ccnd3 ; patient survival ; paired tumor-normal samples ; 11q13 amplification
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Biochemistry and molecular biology
Subject RIV - cooperation	Genetics ; Molecular Biology Institute of Microbiology - Oncology ; Hematology
R&D Projects	GA18-11908S GA ČR - Czech Science Foundation (CSF)
	NV16-29032A GA MZd - Ministry of Health (MZ)
	EF16_019/0000785 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UMG-J - RVO:68378050 ; MBU-M - RVO:61388971
UT WOS	000535587400022
DOI	10.3390/cancers12040792
Annotation	Aberrant regulation of the cell cycle is a typical feature of all forms of cancer. In head and neck squamous cell carcinoma (HNSCC), it is often associated with the overexpression of cyclin D1 (CCND1). However, it remains unclear how CCND1 expression changes between tumor and normal tissues and whether human papillomavirus (HPV) affects differential CCND1 expression. Here, we evaluated the expression of D-type cyclins in a cohort of 94 HNSCC patients of which 82 were subjected to whole genome expression profiling of primary tumors and paired normal mucosa. Comparative analysis of paired samples showed that CCND1 was upregulated in 18% of HNSCC tumors. Counterintuitively, CCND1 was downregulated in 23% of carcinomas, more frequently in HPV-positive samples. There was no correlation between the change in D-type cyclin expression and patient survival. Intriguingly, among the tumors with downregulated CCND1, one-third showed an increase in cyclin D2 (CCND2) expression. On the other hand, one-third of tumors with upregulated CCND1 showed a decrease in CCND2. Collectively, we have shown that CCND1 was frequently downregulated in HNSCC tumors. Furthermore, regardless of the HPV status, our data suggested that a change in CCND1 expression was alleviated by a compensatory change in CCND2 expression.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2021
Electronic address	https://www.mdpi.com/2072-6694/12/4/792

Number of the records: 1