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Plasma treatment in air at atmospheric pressure that enables reagent-free covalent immobilization of biomolecules on polytetrafluoroethylene (PTFE)
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SYSNO ASEP 0524475 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Plasma treatment in air at atmospheric pressure that enables reagent-free covalent immobilization of biomolecules on polytetrafluoroethylene (PTFE) Author(s) Bilek, M. M. M. (AU)
Vandrovcová, Marta (FGU-C) RID, ORCID
Shelemin, A. (CZ)
Kuzminova, A. (CZ)
Kylián, O. (CZ)
Biederman, H. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCID
Weiss, A. S. (AU)Article number 146128 Source Title Applied Surface Science. - : Elsevier - ISSN 0169-4332
Roč. 518, Jul 15 (2020)Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords dielectric barrier discharge (DBD) ; atmospheric pressure plasma ; polytetrafluoroethylene (PTFE) ; biomolecule functionalisation ; protein immobilization ; bovine serum albumin (BSA) ; tropoelastin Subject RIV EI - Biotechnology ; Bionics OECD category Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction) R&D Projects GBP108/12/G108 GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 000531746300005 EID SCOPUS 85082704998 DOI 10.1016/j.apsusc.2020.146128 Annotation Covalent immobilization of biomolecules to surfaces is desirable in applications in biomedicine and biotechnology, such as biosensors, protein microarrays and implantable biomedical devices. Surface-embedded radicals in polymers, produced by plasma immersion ion implantation, are known to covalently immobilize biomolecules directly from buffer without additional reagents. Here we explore the prospects for creating a surface activated for direct covalent immobilization using a dielectric barrier discharge in air at atmospheric pressure, eliminating the need for vacuum and gas feed systems. We find that a surface activation process at atmospheric pressure in air can activate polytetrafluoroethylene (PTFE) in order to achieve reagent-free covalent immobilization of biomolecules. The presence of surface immobilized protein was verified by X-ray photoelectron spectroscopy (XPS), and its covalent immobilization was demonstrated by resilience to rigorous SDS washing at elevated temperature. Time course immobilization studies show that the covalent coupling capability of the activated surfaces is retained for several days. Proof-of-concept cell assays with immobilized tropoelastin demonstrate the technique’s ability to present functional cell binding molecules for the production of truly bioactive surfaces. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2021 Electronic address https://doi.org/10.1016/j.apsusc.2020.146128
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