Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)

Horčičková, Michaela; Čondlová, Šárka; Holubová, Nikola; Sak, Bohumil; Květoňová, Dana; Hlásková, Lenka; Konečný, R.; Sedláček, F.; Clark, M.; Giddings, C.; McEvoy, J.; Kváč, Martin

doi:https://dx.doi.org/10.1017/S0031182018001142

Number of the records: 1

Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)

1.

SYSNO ASEP	0519676
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)
Author(s)	Horčičková, Michaela (BC-A)_ORCID Čondlová, Šárka (BC-A)_ORCID Holubová, Nikola (BC-A)_{SAI, ORCID} Sak, Bohumil (BC-A)_{RID, ORCID} Květoňová, Dana (BC-A)_SAI Hlásková, Lenka (BC-A)_SAI Konečný, R. (CZ) Sedláček, F. (CZ) Clark, M. (US) Giddings, C. (US) McEvoy, J. (US) Kváč, Martin (BC-A)_{RID, RID, ORCID}
Number of authors	12
Source Title	Parasitology. - : Cambridge University Press - ISSN 0031-1820 Roč. 146, č. 2 (2019), s. 220-233
Number of pages	4 s.
Publication form	Print - P
Language	eng - English
Country	GB - United Kingdom
Keywords	boars sus-scrofa ; molecular characterization ; clethrionomys-glareolus ; giardia spp. ; wild rodents ; c-muris ; parvum ; prevalence ; genotypes ; suis ; Experimental infection ; molecular analyses ; oocyst size ; phylogeny ; Rodentia ; voles
Subject RIV	GJ - Animal Vermins ; Diseases, Veterinary Medicine
OECD category	Veterinary science
R&D Projects	LTAUSA17165 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	BC-A - RVO:60077344
UT WOS	000458677800010
EID SCOPUS	85049975640
DOI	10.1017/S0031182018001142
Annotation	Fecal samples from wild-caught common voles (n = 328) from 16 locations in the Czech Republic were screened for Cryptosporidium by microscopy and PCR/sequencing at loci coding small-subunit rRNA, Cryptosporidium oocyst wall protein, actin and 70 kDa heat shock protein. Cryptosporidium infections were detected in 74 voles (22.6%). Rates of infection did not differ between males and females nor between juveniles and adults. Phylogenetic analysis revealed the presence of eight Cryptosporidium species/genotypes including two new species, C. alticolis and C. microti. These species from wild-caught common voles were able to infect common and meadow voles under experimental conditions, with a prepatent period of 3-5 days post-infection (DPI), but they were not infectious for various other rodents or chickens. Meadow voles lost infection earlier than common voles (11-14 vs 13-16 DPI) and had significantly lower infection intensity. Cryptosporidium alticolis infects the anterior small intestine and has larger oocysts (5.4 x 4.9 mu m), whereas C. microti infects the large intestine and has smaller oocysts (4.3 x 4.1 mu m). None of the rodents developed clinical signs of infection. Genetic and biological data support the establishment of C. alticolis and C. microti as separate species of the genus Cryptosporidium.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2020
Electronic address	https://www.cambridge.org/core/journals/parasitology/article/diversity-of-cryptosporidium-in-common-voles-and-description-of-cryptosporidium-alticolis-sp-n-and-cryptosporidium-microti-sp-n-apicomplexa-cryptosporidiidae/9667CA24CCAE50F331A284F0C58F861F

Number of the records: 1