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CKS1 Germ Line Exclusion Is Essential for the Transition from Meiosis to Early Embryonic Development
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SYSNO ASEP 0512107 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title CKS1 Germ Line Exclusion Is Essential for the Transition from Meiosis to Early Embryonic Development Author(s) Ellederová, Zdeňka (UZFG-Y) RID, ORCID
del Rincon, S. (US)
Končická, Markéta (UZFG-Y) ORCID
Šušor, Andrej (UZFG-Y) RID, ORCID
Kubelka, Michal (UZFG-Y) RID, ORCID
Sun, D. (US)
Spruck, C. (US)Article number UNSP e00590-18 Source Title Molecular and Cellular Biology. - : American Society for Microbiology - ISSN 0270-7306
Roč. 39, č. 13 (2019)Number of pages 18 s. Publication form Online - E Language eng - English Country US - United States Keywords CKS ; cyclin dependent kinases ; developmental biology Subject RIV EB - Genetics ; Molecular Biology OECD category Cell biology R&D Projects LO1609 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF15_003/0000460 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA15-22765S GA ČR - Czech Science Foundation (CSF) GA18-19395S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 UT WOS 000471243000005 EID SCOPUS 85068118528 DOI 10.1128/MCB.00590-18 Annotation Cell division cycle (cdc) kinase subunit (CKS) proteins bind cyclin-dependent kinases (CDKs) and play important roles in cell division control and development, though their precise molecular functions are not fully understood. Mammals express two closely related paralogs called CKS1 and CKS2, but only CKS2 is expressed in the germ line, indicating that it is solely responsible for regulating CDK functions in meiosis. Using cks2(-/-) knockout mice, we show that CKS2 is a crucial regulator of maturation-promoting factor (MPF, CDK1-cyclin A/B) activity in meiosis. cks2(-/-) oocytes display reduced and delayed MPF activity during meiotic progression, leading to defects in germinal vesicle breakdown (GVBD), anaphase-promoting complex/cyclosome (APC/C) activation, and meiotic spindle assembly. cks2(-/-) germ cells express significantly reduced levels of the MPF components CDK1 and cyclins A1/B1. Additionally, injection of MPF plus CKS2, but not MPF alone, restored normal GVBD in cks2(-/- )oocytes, demonstrating that GVBD is driven by a CKS2-dependent function of MPF. Moreover, we generated cks2(cks1/cks1)( )knock-in mice and found that CKS1 can compensate for CKS2 in meiosis in vivo, but homozygous embryos arrested development at the 2- to 5-cell stage. Collectively, our results show that CKS2 is a crucial regulator of MPF functions in meiosis and that its paralog, CKS1, must be excluded from the germ line for proper embryonic development. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2020 Electronic address https://mcb.asm.org/content/39/13/e00590-18
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