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Evaluation of collagen/hydroxyapatite electrospun layers loaded with vancomycin, gentamicin and their combination: Comparison of release kinetics, antimicrobial activity and cytocompatibility
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SYSNO ASEP 0511568 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Evaluation of collagen/hydroxyapatite electrospun layers loaded with vancomycin, gentamicin and their combination: Comparison of release kinetics, antimicrobial activity and cytocompatibility Author(s) Suchý, Tomáš (USMH-B) RID, ORCID, SAI
Šupová, Monika (USMH-B) RID, ORCID, SAI
Sauerová, P. (CZ)
Kalbáčová, M.H. (CZ)
Klapková, E. (CZ)
Pokorný, M. (CZ)
Horný, L. (CZ)
Závora, J. (CZ)
Ballay, R. (CZ)
Denk, František (USMH-B) ORCID
Sojka, M. (CZ)
Vištejnová, L. (CZ)Source Title European Journal of Pharmaceutics and Biopharmaceutics. - : Elsevier - ISSN 0939-6411
Roč. 140, JUL 2019 (2019), s. 50-59Number of pages 10 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords Collagen ; Hydroxyapatite ; Vancomycin ; Gentamicin ; Synergistic effect ; Nanofibers ; Nanoparticles ; Antimicrobial activity ; Release kinetics ; Cytocompatibility Subject RIV JJ - Other Materials OECD category Medical engineering R&D Projects TA04010330 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Method of publishing Limited access Institutional support USMH-B - RVO:67985891 UT WOS 000470947400006 EID SCOPUS 85065149726 DOI 10.1016/j.ejpb.2019.04.021 Annotation The aim of this study was to develop a biodegradable nanostructured electrospun layer based on collagen (COL), hydroxyapatite nanoparticles (HA), vancomycin hydrochloride (V), gentamicin sulphate (G) and their combination (VG) for the treatment of prosthetic joint infections and the prevention of infection during the joint replacement procedure. COL/HA layers containing different amounts of HA (0, 5 and 15 wt%) were tested for the in vitro release kinetics of antibiotics, antimicrobial activity against MRSA, gentamicin-resistant Staphylococcus epidermidis and Enterococcus faecalis isolates and cytocompatibility using SAOS-2 bone-like cells. The results revealed that the COL/HA layers released high concentrations of vancomycin and gentamicin for 21 days and performed effectively against the tested clinically-relevant bacterial isolates. The presence of HA in the collagen layers was found not to affect the release kinetics of the vancomycin from the layers loaded only with vancomycin or its combination with gentamicin. Conversely, the presence of HA slowed down the release of gentamicin from the COL/HA layers loaded with gentamicin and its combination with vancomycin. The combination of both antibiotics exerted a positive effect on the prolongation of the conversion of vancomycin into its degradation products. All the layers tested with different antibiotics exhibited potential antibacterial activity with respect to both the tested staphylococci isolates and enterococci. The complemental effect of vancomycin was determined against both gentamicin-resistant Staphylococcus epidermidis and Enterococcus faecalis in contrast to the application of gentamicin as a single agent. This combination was also found to be more effective against MRSA than is vancomycin as a single agent. Importantly, this combination of vancomycin and gentamicin in the COL/HA layers exhibited sufficient cytocompatibility to SAOS-2, which was independent of the HA content. Conversely, only gentamicin caused the death of SAOS-2 independently of HA content and only vancomycin stimulated SAOS-2 behaviour with an increased concentration of HA in the COL/HA layers. In conclusion, COL/HA layers with 15 wt% of HA impregnated with vancomycin or with a combination of vancomycin and gentamicin offer a promising treatment approach and the potential to prevent infection during the joint replacement procedures. Workplace Institute of Rock Structure and Mechanics Contact Iva Švihálková, svihalkova@irsm.cas.cz, Tel.: 266 009 216 Year of Publishing 2020 Electronic address https://www.sciencedirect.com/science/article/pii/S0939641118314292?via%3Dihub
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