Lipopolysaccharides from Microcystis Cyanobacteria-Dominated Water Bloom and from Laboratory Cultures Trigger Human Immune Innate Response

Moosova, Zdena; Šindlerová, Lenka; Ambrůzová, Barbora; Ambrožová, Gabriela; Vašíček, Ondřej; Velki, M.; Babica, P.; Kubala, Lukáš

doi:https://dx.doi.org/10.3390/toxins11040218

Number of the records: 1

Lipopolysaccharides from Microcystis Cyanobacteria-Dominated Water Bloom and from Laboratory Cultures Trigger Human Immune Innate Response

1.

SYSNO ASEP	0509790
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Lipopolysaccharides from Microcystis Cyanobacteria-Dominated Water Bloom and from Laboratory Cultures Trigger Human Immune Innate Response
Author(s)	Moosova, Zdena (BFU-R) Šindlerová, Lenka (BFU-R)_{RID, ORCID} Ambrůzová, Barbora (BFU-R) Ambrožová, Gabriela (BFU-R)_RID Vašíček, Ondřej (BFU-R)_{ORCID, RID} Velki, M. (HR) Babica, P. (CZ) Kubala, Lukáš (BFU-R)_{RID, ORCID}
Number of authors	8
Article number	218
Source Title	Toxins. - : MDPI - ISSN 2072-6651 Roč. 11, č. 4 (2019)
Number of pages	16 s.
Publication form	Online - E
Language	eng - English
Country	CH - Switzerland
Keywords	escherichia-coli lipopolysaccharide ; oscillatoria sp lipopolysaccharide ; heterotrophic bacteria ; toxins microcystins ; aeruginosa
Subject RIV	GM - Food Processing
OECD category	Food and beverages
R&D Projects	GA16-24949S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	BFU-R - RVO:68081707
UT WOS	000467299600036
DOI	10.3390/toxins11040218
Annotation	Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide. Here, the biological activities of lipopolysaccharide (LPS) isolated from Microcystis aeruginosa, the most prominent cyanobacteria in water bloom, were studied. LPS was isolated from complex environmental water bloom samples dominated by M. aeruginosa, and from laboratory cultures of non-axenic as well as axenic M. aeruginosa strains PCC7806 and HAMBI/UHCC130. Employing human blood-based in vitro tests, the LPS isolated from complex water bloom revealed the priming of both major blood phagocyte population monocytes and polymorphonuclear leukocytes documented by the increased surface expression of CD11b and CD66b. This was accompanied by a water bloom LPS-mediated dose-dependent induction of tumor necrosis factor , interleukin-1, and interleukin-6 production. In accordance with its priming effects, water bloom LPS induced significant activation of p38 and ERK1/2 kinases, as well as NF-B phosphorylation, in isolated polymorphonuclear leukocytes. Interestingly, the pro-inflammatory potential of LPS from the axenic strain of M. aeruginosa was not lower compared to that of LPS isolated from non-axenic strains. In contrast to the biological activity, water bloom LPS revealed almost twice higher pyrogenicity levels compared to Escherichia coli LPS, as analyzed by the PyroGene test. Moreover, LPS from the non-axenic culture exhibited higher endotoxin activity in comparison to LPS from axenic strains. Taking the above findings together, M. aeruginosa LPS can contribute to the health risks associated with contamination by complex water bloom mass.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2020
Electronic address	https://www.mdpi.com/2072-6651/11/4/218/pdf

Number of the records: 1