Cochlear ablation in neonatal rats disrupts inhibitory transmission in the medial nucleus of the trapezoid body

0508226 - ÚEM 2020 RIV IE eng J - Journal Article
Hrušková, Bohdana - Trojanová, Johana - Králíková, Michaela - Melichar, Adolf - Suchánková, Štěpánka - Bartošová, Jolana - Burianová, Jana - Popelář, Jiří - Syka, Josef - Tureček, Rostislav
Cochlear ablation in neonatal rats disrupts inhibitory transmission in the medial nucleus of the trapezoid body.
Neuroscience Letters. Roč. 699, APR (2019), s. 145-150. ISSN 0304-3940. E-ISSN 1872-7972
R&D Projects: GA ČR(CZ) GA16-17823S; GA ČR(CZ) GA14-28334S
Institutional support: RVO:68378041
Keywords : Cochlear ablation * GABA * glycine * MNTB * auditory
OECD category: Neurosciences (including psychophysiology
Impact factor: 2.274, year: 2019
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/abs/pii/S030439401930076X?via%3Dihub

Inhibitory circuits in the auditory brainstem undergo multiple postnatal changes that are both activity-dependent and activity-independent. We tested to see if the shift from GABA- to glycinergic transmission, which occurs in the rat medial nucleus of the trapezoid body (MNTB) around the onset of hearing, depends on sound-evoked neuronal activity. We prevented the activity by bilateral cochlear ablations in early postnatal rats and studied ionotropic GABA and glycine receptors in MNTB neurons after hearing onset. The removal of the cochlea decreased responses of GABA(A) and glycine receptors to exogenous agonists as well as the amplitudes of inhibitory postsynaptic currents. The reduction was accompanied by a decrease in the number of glycine receptor- or vesicular GABA transporter-immunopositive puncta. Furthermore, the ablations markedly affected the switch in presynaptic GABA(A) to glycine receptors. The increase in the expression of postsynaptic glycine receptors and the shift in inhibitory transmitters were not prevented. The results suggest that inhibitory transmission in the MNTB is subject to multiple developmental signals and support the idea that auditory experience plays a role in the maturation of the brainstem glycinergic circuits.
Permanent Link: http://hdl.handle.net/11104/0299197