Assessment of somaclonal variation in indirect morphogenesis-derived plants of Arracacia xanthorrhiza

0507911 - ÚEB 2021 RIV BR eng J - Journal Article
Vítámvás, J. - Viehmannová, I. - Čepková Hlásná, P. - Mrhalová, H. - Eliášová, Kateřina
Assessment of somaclonal variation in indirect morphogenesis-derived plants of Arracacia xanthorrhiza.
Pesquisa Agropecuaria Brasileira. Roč. 54, č. 2019 (2019), č. článku e00301. ISSN 0100-204X. E-ISSN 1678-3921
Grant - others:Ministerstvo zemědělství(CZ) RO0418
Institutional support: RVO:61389030
Keywords : nuclear-dna content * somatic embryogenesis * genetic stability * shoot organogenesis * sequence * cultures * rapd * arracacha * ISSR * plant breeding * ploidy level * somatic embryogenesis
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 0.644, year: 2019
Method of publishing: Open access
http://dx.doi.org/10.1590/S1678-3921.pab2019.v54.00301

The objective of this work was to induce and detect somaclonal variation in arracacha (Arracacia xanthorrhiza) plants regenerated via indirect morphogenesis, in order to evaluate the potential of this technique to produce new genotypes for breeding purposes of this crop. Calli were induced from petiole segments on Murashige & Skoog (MS) medium supplied with 0.1 mg L-1 2,4-dichlorophenoxyacetic acid. The regeneration of plants via indirect morphogenesis was carried out on half-strength MS medium without plant growth regulators. Fifteen randomly chosen plants were subjected to flow cytometry and “inter-simple sequence repeat” (ISSR) analysis. Ploidy level remained stable in all tested regenerants (2n=4x=44), with no changes in the genome. Eighteen ISSR primers produced a total of 1,584 fragments in all samples. Two ISSR primers produced four polymorphic fragments in 26.7% of the tested samples. Somaclonal variation in arracacha is a result of plant regeneration via indirect morphogenesis and can be detected by ISSR markers.
Permanent Link: http://hdl.handle.net/11104/0298876