In situ in vivo radiolabeling of polymer-coated hydroxyapatite nanoparticles to track their biodistribution in mice

0503770 - ÚMCH 2020 RIV NL eng J - Journal Article
Lobaz, Volodymyr - Konefal, Rafal - Pánek, Jiří - Vlk, M. - Kozempel, J. - Petřík, M. - Nový, Z. - Gurská, S. - Znojek, P. - Štěpánek, Petr - Hrubý, Martin
In situ in vivo radiolabeling of polymer-coated hydroxyapatite nanoparticles to track their biodistribution in mice.
Colloids and Surfaces B-Biointerfaces. Roč. 179, 1 July (2019), s. 143-152. ISSN 0927-7765. E-ISSN 1873-4367
R&D Projects: GA MZd(CZ) NV16-30544A
Institutional support: RVO:61389013
Keywords : bone-seeking agent * hydroxybisphosphonate * polyoxazoline
OECD category: Polymer science
Impact factor: 4.389, year: 2019
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0927776519302061?via%3Dihub

The imaging of healthy tissues and solid tumors benefits from the application of nanoparticle probes with altered pharmacokinetics, not available to low molecular weight compounds. However, the distribution and accumulation of nanoprobes in vivo typically take at least tens of hours to be efficient. For nanoprobes bearing a radioactive label, this is contradictory to the requirement of minimizing the radiation dose for patients by using as-short-as-feasible half-life radionuclides in diagnostics. Thus, we developed a two-stage diagnostic concept for monitoring long-lasting targeting effects with short-lived radioactive labels using bone-mimicking biocompatible polymer-coated and colloidally fully stabilized hydroxyapatite nanoparticles (HAP NPs) and bone-seeking radiopharmaceuticals. Within the pretargeting stage, the nonlabeled nanoparticles are allowed to circulate in the blood. Afterward, 99mTc-1-hydroxyethylidene-1.1-diphosphonate (99mTc-HEDP) is administered intravenously for in situ labeling of the nanoparticles and subsequent single-photon emission computed tomography/computed tomography (SPECT/CT) visualization. The HAP NPs, stabilized with tailored hydrophilic polymers, are not cytotoxic in vitro, as shown by several cell lines. The polymer coating prolongs the circulation of HAP NPs in the blood. The nanoparticles were successfully labeled in vivo with 99mTc-HEDP, 1 and 24 h after injection, and they were visualized by SPECT/CT over time in healthy mice.
Permanent Link: http://hdl.handle.net/11104/0295813