Endogenous Chemiluminescence from Germinating Arabidopsis Thaliana Seeds

0499899 - ÚFE 2019 RIV GB eng J - Journal Article
Saeidfirozeh, H. - Shafiekhani, A. - Cifra, Michal - Masoudi, A.A.
Endogenous Chemiluminescence from Germinating Arabidopsis Thaliana Seeds.
Scientific Reports. Roč. 8, č. 1 (2018), č. článku 16231. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA ČR(CZ) GA13-29294S
Grant - others:AV ČR(CZ) SAV-18-11
Program: Bilaterální spolupráce
Institutional support: RVO:67985882
Keywords : chemiluminescence * Arabidopsis thaliana * Photons
OECD category: Electrical and electronic engineering
Impact factor: 4.011, year: 2018

t is well known that all biological systems which undergo oxidative metabolism or oxidative stress generate a small amount of light. Since the origin of excited states producing this light is generally accepted to come from chemical reactions, the term endogenous biological chemiluminescence is appropriate. Apart from biomedicine, this phenomenon has potential applications also in plant biology and agriculture like monitoring the germination rate of seeds. While chemiluminescence capability to monitor germination has been measured on multiple agriculturally relevant plants, the standard model plant Arabidopsis thaliana has not been analyzed for this process so far. To fill in this gap, we demonstrate here on A. thaliana that the intensity of endogenous chemiluminescence increases during the germination stage. We showed that the chemiluminescence intensity increases since the second day of germination, but reaches a plateau on the third day, in contrast to other plants germinating from larger seeds studied so far. We also showed that intensity increases after topical application of hydrogen peroxide in a dose-dependent manner. Further, we demonstrated that the entropy of the chemiluminescence time series is similar to random Poisson signals. Our results support a notion that metabolism and oxidative reactions are underlying processes which generate endogenous biological chemiluminescence. Our findings contribute to novel methods for non-invasive and label-free sensing of oxidative processes in plant biology and agriculture
Permanent Link: http://hdl.handle.net/11104/0292088