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High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging
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SYSNO ASEP 0497594 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging Author(s) Turtaev, S. (DE)
Leite, I. T. (DE)
Altwegg-Boussac, T. (GB)
Pakan, J.M.P. (GB)
Rochefort, N.L. (GB)
Čižmár, Tomáš (UPT-D) RID, ORCID, SAINumber of authors 6 Article number 92 Source Title Light-Science & Applications. - : Springer - ISSN 2047-7538
Roč. 7, č. 1 (2018)Number of pages 8 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords optical-fiber ; focusing light ; transmission ; amplitude Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) R&D Projects EF15_003/0000476 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 UT WOS 000450708600003 EID SCOPUS 85057042186 DOI 10.1038/s41377-018-0094-x Annotation Progress in neuroscience relies on new techniques for investigating the complex dynamics of neuronal networks. An ongoing challenge is to achieve minimally invasive and high-resolution observations of neuronal activity in vivo inside deep brain areas. Recently introduced methods for holographic control of light propagation in complex media enable the use of a hair-thin multimode optical fibre as an ultranarrow imaging tool. Compared to endoscopes based on graded-index lenses or fibre bundles, this new approach offers a footprint reduction exceeding an order of magnitude, combined with a significant enhancement in resolution. We designed a compact and high-speed system for fluorescent imaging at the tip of a fibre, achieving a resolution of 1.18 +/- 0.04 mu m across a 50-mu m field of view, yielding 7-kilopixel images at a rate of 3.5 frames/s. Furthermore, we demonstrate in vivo observations of cell bodies and processes of inhibitory neurons within deep layers of the visual cortex and hippocampus of anaesthetised mice. This study paves the way for modern microscopy to be applied deep inside tissues of living animal models while exerting a minimal impact on their structural and functional properties. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2019
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