Number of the records: 1
TATAA courses – 2 Days qPCR for miRNA analysis\n\n
- 1.
SYSNO ASEP 0496888 Document Type U - Organizing Conference, Workshop, Exhibition R&D Document Type W - Uspořádání workshopu R&D Document Type Není vybrán druh dokumentu Title TATAA courses – 2 Days qPCR for miRNA analysis
Author(s) Kubista, Mikael (BTO-N) RID
Valihrach, Lukáš (BTO-N) RID, ORCID
Androvič, Peter (BTO-N)
Rohlová, Eva (BTO-N)Event type W - Workshop Event date 10.05.2018 - 11.05.2018 VEvent location Vestec, BIOCEV Country CZ - Czech Republic Event type WRD Total number of participants 11 Number of foreign participants 5 Language eng - English Keywords qPCR ; quality control ; miRNA analysis Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BTO-N - RVO:86652036 Annotation This course introduces miRNA and its variant forms. Methods to extract and purify microRNA and prepare it for analysis, including quality control. Methods for microRNA expression analysis are presented and compared, to learn how to too choose the best method for each study. Analysis of microRNA data is also discussed, including challenging normalization. The course contains:
Day 1
• MicroRNA background including biogenesis and function
• MicroRNA extraction – sampling, miRNA stability, extraction kit comparison
• Quality Control of miRNA
• MicroRNA and liquid biopsies – challenges of miRNA analysis in biofluids
• Hands-on lab testing of quality and quantity of miRNA
Day 2
• MicroRNA quantification with microarray, nanoString, NGS and RT -qPCR methods
• Comparison of miRNA quantification methods
• Experimental design and data analysis
• RT-qPCR normalization of miRNA data
• Introduction to NGS data analysis
• Hands-on lab of miRNA RT-qPCR analysis including data analysis in Genex
Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2019
Number of the records: 1