Number of the records: 1
Poly(amino acid)-based nanogel by horseradish peroxidase catalyzed crosslinking in an inverse miniemulsion
- 1.
SYSNO ASEP 0489472 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Poly(amino acid)-based nanogel by horseradish peroxidase catalyzed crosslinking in an inverse miniemulsion Author(s) Šálek, Petr (UMCH-V) RID, ORCID
Dvořáková, Jana (UMCH-V) RID, ORCID
Černoch, Peter (UMCH-V) RID, ORCID
Pavlova, Ewa (UMCH-V) RID
Proks, Vladimír (UMCH-V) RID, ORCIDSource Title Colloid and Polymer Science - ISSN 0303-402X
Roč. 296, č. 6 (2018), s. 995-1003Number of pages 9 s. Language eng - English Country DE - Germany Keywords enzymatic crosslinking ; inverse miniemulsion ; nanogel Subject RIV CD - Macromolecular Chemistry OECD category Polymer science R&D Projects GA16-02702S GA ČR - Czech Science Foundation (CSF) Institutional support UMCH-V - RVO:61389013 UT WOS 000431929900001 EID SCOPUS 85046862434 DOI 10.1007/s00396-018-4318-7 Annotation We present an investigation of horseradish peroxidase (HRP)/H2O2-mediated crosslinking in an inverse miniemulsion for the successful preparation of a stable colloidal nanogel from a poly(amino acid)-based polymer precursor. The precursor was obtained by the aminolysis of polysuccinimide with aminoethan-2-ol and tyramine, resulting in a poly(α,β-N-(2-hydroxyethyl)-D,L-aspartamide-co-N-(2-(4-hydroxyphenyl)ethyl)-D,L-aspartamide) polymer (PHEA-Tyr). Various concentrations of the PHEA-Tyr in aqueous solution with HRP were emulsified in the presence of cyclohexane and SPAN 80. The addition of a hydrogen peroxide solution induced crosslinking between the polymer chains via the phenol groups (Tyr) and targeted nanogel formation. The hydrodynamic radii (R h 0), mean size documented by hydrodynamic radius (R h ), and morphology of the nanoparticles were investigated by dynamic light scattering (DLS) measurements, nanoparticle tracking analysis (NTA), and cryogenic transmission electron microscopy (cryo-TEM). It was found out that nanoparticle radius, morphology, and architecture of the nanogel could be regulated by the initial concentration of the precursor. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2019
Number of the records: 1