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Poly(amino acid)-based nanogel by horseradish peroxidase catalyzed crosslinking in an inverse miniemulsion
- 1.0489472 - ÚMCH 2019 RIV DE eng J - Journal Article
Šálek, Petr - Dvořáková, Jana - Černoch, Peter - Pavlova, Ewa - Proks, Vladimír
Poly(amino acid)-based nanogel by horseradish peroxidase catalyzed crosslinking in an inverse miniemulsion.
Colloid and Polymer Science. Roč. 296, č. 6 (2018), s. 995-1003. ISSN 0303-402X. E-ISSN 1435-1536
R&D Projects: GA ČR(CZ) GA16-02702S
Institutional support: RVO:61389013
Keywords : enzymatic crosslinking * inverse miniemulsion * nanogel
OECD category: Polymer science
Impact factor: 1.906, year: 2018
We present an investigation of horseradish peroxidase (HRP)/H2O2-mediated crosslinking in an inverse miniemulsion for the successful preparation of a stable colloidal nanogel from a poly(amino acid)-based polymer precursor. The precursor was obtained by the aminolysis of polysuccinimide with aminoethan-2-ol and tyramine, resulting in a poly(α,β-N-(2-hydroxyethyl)-D,L-aspartamide-co-N-(2-(4-hydroxyphenyl)ethyl)-D,L-aspartamide) polymer (PHEA-Tyr). Various concentrations of the PHEA-Tyr in aqueous solution with HRP were emulsified in the presence of cyclohexane and SPAN 80. The addition of a hydrogen peroxide solution induced crosslinking between the polymer chains via the phenol groups (Tyr) and targeted nanogel formation. The hydrodynamic radii (R h 0), mean size documented by hydrodynamic radius (R h ), and morphology of the nanoparticles were investigated by dynamic light scattering (DLS) measurements, nanoparticle tracking analysis (NTA), and cryogenic transmission electron microscopy (cryo-TEM). It was found out that nanoparticle radius, morphology, and architecture of the nanogel could be regulated by the initial concentration of the precursor.
Permanent Link: http://hdl.handle.net/11104/0284164
Number of the records: 1