Number of the records: 1  

Diversity of Entamoeba spp. in African great apes and humans: an insight from Illumina MiSeq high-throughput sequencing

  1. 1.
    0489324 - ÚBO 2019 RIV AU eng J - Journal Article
    Vlčková, Klára - Kreisinger, J. - Pafčo, B. - Čížková, Dagmar - Tagg, N. - Hehl, A. B. - Modrý, David
    Diversity of Entamoeba spp. in African great apes and humans: an insight from Illumina MiSeq high-throughput sequencing.
    International Journal for Parasitology. Roč. 48, č. 7 (2018), s. 519-530. ISSN 0020-7519. E-ISSN 1879-0135
    Institutional support: RVO:68081766 ; RVO:60077344
    Keywords : Entamoeba * Western lowland gorilla * Central chimpanzee * Humans * Metabarcoding * Diversity * Mixed infections * Entamoeba histolytica
    OECD category: Parasitology; Parasitology (BC-A)
    Impact factor: 3.478, year: 2018

    Understanding the complex Entamoeba communities in the mammalian intestine has been, to date, complicated by the lack of a suitable approach for molecular detection of multiple variants co-occurring in mixed infections. Here, we report on the application of a high throughput sequencing approach based on partial 18S rDNA using the Illumina MiSeq platform. We describe, to our knowledge, for the first time, the Entamoeba communities in humans, free-ranging western lowland gorillas and central chimpanzees living in the Dja Faunal Reserve in Cameroon. We detected 36 Entamoeba haplotypes belonging to six haplotype clusters, containing haplotypes possessing high and low host specificity. Most of the detected haplotypes belonged to commensal Entamoeba, however, the pathogenic species (Entamoeba histolytica and Entamoeba nuttalli) were also detected. We observed that some Entamoeba haplotypes are shared between humans and other hosts, indicating their zoonotic potential. The findings are important not only for understanding the epidemiology of amoebiasis in humans in rural African localities, but also in the context of wild great ape conservation.
    Permanent Link: http://hdl.handle.net/11104/0283766

     
     
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.