Monitoring lipid production in yeast using SEM and Raman spectroscopy

0481587 - ÚPT 2018 RIV HR eng C - Conference Paper (international conference)
Hrubanová, Kamila - Krzyžánek, Vladislav - Samek, Ota - Šiler, Martin - Zemánek, Pavel - Hároniková, A. - Marová, I.
Monitoring lipid production in yeast using SEM and Raman spectroscopy.
13th Multinational Congress on Microscopy: Book of Abstracts. Zagreb: Ruder Bošković Institute, Croatian Microscopy Society, 2017 - (Gajović, A.; Weber, I.; Kovačević, G.; Čadež, V.; Šegota, S.; Peharec Štefanić, P.; Vidoš, A.), s. 308-309. ISBN 978-953-7941-19-2.
[Multinational Congress on Microscopy /13./. Rovinj (HR), 24.09.2017-29.09.2017]
R&D Projects: GA ČR(CZ) GA15-20645S; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : yeast * lipid bodies * SEM * Raman spectroscopy
OECD category: Electrical and electronic engineering

The main goal of our project is the development of specialized instrumentation and methodology to monitor metabolic states of microorganisms in order to optimize cultivation process for biotechnological applications. Here the attention is given to the oil-producing yeast strain Metschnikowia pulcherrima. The influence of different cultivation parameters (such as the effects of temperature regime and medium composition) on cells can be monitored. In our investigations on yeast cells scanning electron microscopy (SEM) and Raman spectroscopy techniques were used. Here, SEM uses electron beam to gain information about morphology of cells which reflects cells response on the different cultivation conditions. Consequently, Raman spectroscopy was used for the determination of lipids/oil present in the biomass. Thus, our study targets some factors which could lead to efficient industrial production of oil in selected biotechnological production. The yeast strains (Metschnikowia) were cultivated in medium containing different ratios of C/N (glycerol/yeast extract and/or ammonium sulphate) during a time period of two weeks. On the different days of cultivation, the cells were collected by centrifugation (2000 rpm, 1 min.) and analysed. SEM - the samples of yeast were cultivated as mentioned above and observed by cryo scanning electron microscopy (cryo-SEM).
Permanent Link: http://hdl.handle.net/11104/0277136