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The therapeutic potential of three-dimensional multipotent mesenchymal stromal cell spheroids
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SYSNO ASEP 0477186 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The therapeutic potential of three-dimensional multipotent mesenchymal stromal cell spheroids Author(s) Petrenko, Yuriy (UEM-P) RID, ORCID
Syková, Eva (UEM-P) RID
Kubinová, Šárka (UEM-P) RID, ORCIDSource Title Stem Cell Research & Therapy. - : BioMed Central
Roč. 8, apr 26 (2017), s. 94Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords multipotent mesenchymal stromal cells ; three-dimensional spheroids ; clinical-grade manufacturing Subject RIV FH - Neurology OECD category Neurosciences (including psychophysiology R&D Projects LO1309 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA15-01396S GA ČR - Czech Science Foundation (CSF) GA17-03765S GA ČR - Czech Science Foundation (CSF) LM2015064 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEM-P - RVO:68378041 UT WOS 000400138200001 EID SCOPUS 85018836932 DOI 10.1186/s13287-017-0558-6 Annotation The efficiency of clinical trials involving transplantation of multipotent mesenchymal stromal cells (MSCs) is often insufficient due to harsh conditions present within the target tissue including hypoxia, low nutrient supply as well as inflammatory reactions. This indicates the necessity for optimization of cell-based therapy approaches which might include either modification of the cell manufacturing process or specific cell pretreatment procedures prior to transplantation. Recent reports confirm evidence that the aggregation of MSCs into three-dimensional (3D) multicellular spheroids results in enhancement of the overall therapeutic potential of cells, by improving the anti-inflammatory and angiogenic properties, stemness and survival of MSCs after transplantation. Such an MSCs spheroid generation approach may open new opportunities for the enlargement of MSCs applications in clinical research and therapy. However, the unification and optimization of 3D spheroid generation techniques, including the selection of appropriate clinical-grade culture conditions and methods for their large-scale production, are still of great importance. The current review addresses questions regarding therapeutic-associated properties of 3D multicellular MSCs spheroids in vitro and during preclinical animal studies, with special attention to the possibilities of translating these research achievements toward further clinical manufacturing and applications. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2018
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