Analysis of proteins and peptides by electromigration methods in microchips

0474816 - ÚOCHB 2018 RIV DE eng J - Journal Article
Štěpánová, Sille - Kašička, Václav
Analysis of proteins and peptides by electromigration methods in microchips.
Journal of Separation Science. Roč. 40, č. 1 (2017), s. 228-250. ISSN 1615-9306. E-ISSN 1615-9314
R&D Projects: GA ČR(CZ) GA15-01948S
Institutional support: RVO:61388963
Keywords : microchip electrophoresis * microfluidics * peptides * proteins
OECD category: Analytical chemistry
Impact factor: 2.415, year: 2017
http://onlinelibrary.wiley.com/doi/10.1002/jssc.201600962/full

This review presents the developments and applications of microchip electromigration methods in the separation and analysis of peptides and proteins in the period 2011-mid-2016. The developments in sample preparation and preconcentration, microchannel material, and surface treatment are described. Separations by various microchip electromigration methods (zone electrophoresis in free and sieving media, affinity electrophoresis, isotachophoresis, isoelectric focusing, electrokinetic chromatography, and electrochromatography) are demonstrated. Advances in detection methods are reported and novel applications in the areas of proteomics and peptidomics, quality control of peptide and protein pharmaceuticals, analysis of proteins and peptides in biomatrices, and determination of physicochemical parameters are shown.
Permanent Link: http://hdl.handle.net/11104/0271771