Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1

Deloizy, Ch.; Bouguyon, E.; Fossum, E.; Šebo, Peter; Osička, Radim; Bole, A.; Pierres, M.; Biacchesi, S.; Dalod, M.; Bogen, B.; Bertho, N.; Schwartz-Cornil, I.

doi:https://dx.doi.org/10.1016/j.dci.2016.06.015

Number of the records: 1

Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1

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SYSNO ASEP	0472521
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1
Author(s)	Deloizy, Ch. (FR) Bouguyon, E. (FR) Fossum, E. (NO) Šebo, Peter (MBU-M)_{RID, ORCID} Osička, Radim (MBU-M)_{RID, ORCID} Bole, A. (FR) Pierres, M. (FR) Biacchesi, S. (FR) Dalod, M. (FR) Bogen, B. (NO) Bertho, N. (FR) Schwartz-Cornil, I. (FR)
Source Title	Developmental and Comparative Immunology. - : Elsevier - ISSN 0145-305X Roč. 65, December 2016 (2016), s. 31-40
Number of pages	10 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Mononuclear phagocytes ; Dendritic cells ; Pig model
Subject RIV	EE - Microbiology, Virology
R&D Projects	GA15-09157S GA ČR - Czech Science Foundation (CSF)
Institutional support	MBU-M - RVO:61388971
UT WOS	000384388400004
EID SCOPUS	84977134494&
DOI	10.1016/j.dci.2016.06.015
Annotation	ig is a domestic species of major importance in the agro-economy and in biomedical research. Mononuclear phagocytes (MNP) are organized in subsets with specialized roles in the orchestration of the immune response and new tools are awaited to improve MNP subset identification in the pig. We cloned pig CD11c cDNA and generated a monoclonal antibody to pig CD11c which showed a pattern of expression by blood and skin MNP subsets similar to humans. We also developed a porcine XCL1-mCherry dimer which specifically reacted with the XCR1-expressing dendritic cell subset of the type 1 lineage in blood and skin. These original reagents will allow the efficient identification of pig MNP subsets to study their role in physiological and pathological processes and also to target these cells in novel intervention and vaccine strategies for veterinary applications and preclinical evaluations.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2017

Number of the records: 1