The effect of lanthanides on photosynthesis, growth, and chlorophyll profile of the green alga Desmodesmus quadricauda

0467806 - MBÚ 2017 RIV NL eng J - Journal Article
Řezanka, Tomáš - Kaineder, K. - Mezricky, D. - Řezanka, M. - Bišová, Kateřina - Zachleder, Vilém - Vítová, Milada
The effect of lanthanides on photosynthesis, growth, and chlorophyll profile of the green alga Desmodesmus quadricauda.
Photosynthesis Research. Roč. 130, 1-3 SI (2016), s. 335-346. ISSN 0166-8595. E-ISSN 1573-5079
R&D Projects: GA MŠMT(CZ) LO1416; GA ČR GA14-00227S
Institutional support: RVO:61388971
Keywords : Chlorophyll * Green algae * High-resolution electrospray mass spectrometry
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.864, year: 2016

Lanthanides (La, Gd, Nd, Ce) accumulated in the green alga Desmodesmus quadricauda but their intracellular localizations were distinctly different: lanthanum and gadolinium were localized in cytoplasm, while neodymium and cerium were in the chloroplast. The effect of lanthanum and neodymium, as representatives of these two groups, on growth, chlorophyll content and photosynthetic rate at different light intensities was studied. At the lowest light intensity used (50 A mu mol photons m(-2) s(-1)), in the presence of lanthanides (Nd), growth was enhanced by as much as 36 % over lanthanide free control, and the photosynthetic rate increased by up to 300 %. At high light intensities (238, 460, and 750 A mu mol photons m(-2) s(-1)), photosynthetic rate increased markedly, but there was no significant difference between rates in the presence or absence of lanthanides. However, growth, measured as a percentage of dry weight, if compared with lanthanide free control, increased at all light intensities (31, 39, and 20 %, respectively). The total amount of chlorophyll after lanthanide treatment increased by up to 21 % relative to the control culture, mainly due to an increase in the level of chlorophyll b. Addition of lanthanides caused a change in the chlorophyll a/b ratio from 4.583 in control cultivation, to 1.05. Possible mechanisms of lanthanide-induced photosynthetic change, alterations in photosynthetic structures, and increases in growth are discussed and compared with findings in higher plants. The hypothesis that the lanthanide effect could be due to formation of lanthanide-pheophytins was not confirmed as lanthanide pheophytins were not found in D. quadricauda. Furthermore, we have shown that the preferential incorporation of heavy isotopes of magnesium, namely Mg-25 and Mg-26, into chlorophyll during photosynthesis that occurred in controls was diminished in the presence of lanthanides.
Permanent Link: http://hdl.handle.net/11104/0266213