Reduced Levels of Tissue Inhibitors of Metalloproteinases in UVB-Irradiated Corneal Epithelium

0465122 - ÚŽFG 2017 RIV US eng J - Journal Article
Ardan, Taras - Němcová, Lucie - Bohuslavová, Božena - Klezlová, A. - Popelka, Štěpán - Studenovská, Hana - Hrnčiarová, Eva - Čejková, Jitka - Motlík, Jan
Reduced Levels of Tissue Inhibitors of Metalloproteinases in UVB-Irradiated Corneal Epithelium.
Photochemistry and Photobiology. Roč. 92, č. 5 (2016), s. 720-727. ISSN 0031-8655. E-ISSN 1751-1097
R&D Projects: GA ČR GPP302/10/P155; GA MŠMT(CZ) LO1609
Institutional support: RVO:67985904 ; RVO:61389013 ; RVO:68378041
Keywords : tissue inhibitors of metalloproteinases * matrix metalloproteinases
Subject RIV: EB - Genetics ; Molecular Biology; CD - Macromolecular Chemistry (UMCH-V)
Impact factor: 2.121, year: 2016

Tissue inhibitors of metalloproteinases (TIMPs) are the major endogenous regulators of metalloproteinase activity in tissues. TIMPs are able to inhibit activity of all known matrix metalloproteinases (MMPs) and thus participate in controlling extracellular matrix synthesis and degradation. We showed previously elevated expressions of MMPs in the rabbit corneal epithelium upon UVB exposure and suggested that these enzymes might be involved in corneal destruction caused by excessive proteolysis. The aim of this study was to investigate TIMPs in the corneal epithelium after UV irradiation using immunohistochemical and biochemical methods. We found that as compared to control rabbit corneas where relatively high levels of TIMPs were present in the epithelium, repeated irradiation of the cornea with UVB rays (not with UVA rays of similar doses) significantly decreased TIMPs in corneal epithelial cells. The results of this study point to the suggestion that the decrease in TIMPs in the corneal epithelium after UVB irradiation contributes to increased proteolytic activity of MMPs in UVB-irradiated corneal epithelium found previously.
Permanent Link: http://hdl.handle.net/11104/0263823