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Enantiomeric separation of triacylglycerols containing polyunsaturated fatty acids with 18 carbon atoms
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SYSNO ASEP 0464651 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Enantiomeric separation of triacylglycerols containing polyunsaturated fatty acids with 18 carbon atoms Author(s) Řezanka, Tomáš (MBU-M) ORCID
Nedbalová, L. (CZ)
Sigler, Karel (MBU-M) RIDSource Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1467, October (2016), s. 261-269Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords Chiral triacylglycerols ; Enantiomers ; Regioisomers Subject RIV EE - Microbiology, Virology R&D Projects GA14-00227S GA ČR - Czech Science Foundation (CSF) Institutional support MBU-M - RVO:61388971 UT WOS 000385323800022 EID SCOPUS 84978805901 DOI 10.1016/j.chroma.2016.07.006 Annotation Regioisomers and enantiomers of triacylglycerols (TAGs) containing any combination of stearidonic (18:4n-3) and octadecapentaenoic (18:5n-3) acids were prepared by organic synthesis. Gradient polar organic liquid chromatography/high resolution atmospheric pressure chemical ionization-tandem mass spectrometry (NARP-LC/HRMS2-APCI) and chiral liquid chromatography were used for the separation and identification of molecular species of these TAGs. Further, NARP-LC and chiral LC were used to separate natural mixtures of TAGs obtained from the haptophyte alga Coccolithophora sp. cultivated in a salinity range from 7.5 to 60 parts per thousand. The ratio of regioisomers and enantiomers was found to change with increasing salinity of the culture medium. This can be explained by variable activity of acyltransferases in cells exposed to salt stress. (C) 2016 Elsevier B.V. All rights reserved. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2017
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