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Diabetes mellitus negatively affects male reproductive parameters in vivo
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SYSNO ASEP 0462997 Document Type K - Proceedings Paper (Czech conf.) R&D Document Type The record was not marked in the RIV Title Diabetes mellitus negatively affects male reproductive parameters in vivo Author(s) Valášková, Eliška (BTO-N)
Žatecká, Eva (BTO-N)
Pavlínková, Gabriela (BTO-N) RID, ORCID
Bohuslavová, Romana (BTO-N) RID
Dorosh, Andriy (BTO-N) RID
Elzeinová, Fatima (BTO-N) RID
Kubátová, Alena (BTO-N) RID
Margaryan, Hasmik (BTO-N) RID
Pěknicová, Jana (BTO-N) RIDNumber of authors 9 Source Title Book of abstracts of XXIst Symposium of biology and immunology of reproduction. - Praha : Biotechnologický ústav AVČR, 2015 / Pěknicová Jana ; Elzeinová Fatima ; Kubátová Alena
S. 32-32Number of pages 1 s. Action XXIst SYMPOSIUM OF BIOLOGY AND IMMUNOLOGY OF REPRODUCTION WITH INTERNATIONAL PARTICIPATION Event date 14.05.2015-16.05.2015 VEvent location Třešť Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords diabetes melitus ; Sterptozotocin ; apoptosis ; protamination Subject RIV CE - Biochemistry R&D Projects GA P503/12/1834 GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BTO-N - RVO:86652036 Annotation According to the World Health Organization (WHO), 15% of couples in reproductive age suffer from infertility problems, and up to 60% of cases are caused by male factor. This could be caused by genetic background, environmental factors and various diseases, including diabetes mellitus (DM). However, the impact of DM on male fertility is not fully understood. . The aim of this study was to investigate the effects of DM on reproductive parameters and sperm quality, using mouse model. DM (type 1) was induced by Streptozotocin in FVB inbred mouse strain. Mice with blood sugar levels higher than 13.9 mmol/L were considered diabetic. After 4 weeks of diabetes exposure, diabetic males were bred with wild type females and transgenerational effect of DM was assessed. Selected morphological, cellular, and molecular parameters of diabetic males and their male offspring were compared to appropriate controls. There was an increased in sperm fragmentation and abnormalities of sperm morphology in diabetic mice in both generations. An increased staining with apoptotic marker annexin V was also detected in the diabetic groups. Furthermore, a presence of protamines as major sperm nuclear proteins was analysed. Protamine 1 to protamine 2 ratio (P1/P2), a marker of male fertility, was altered in sperms of experimental diabetic animals in both generations. Our findings indicate that DM type 1 negatively affects sperm quality and P1/P2 ratio and this negative effect is transmitted to the progeny Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2017
Number of the records: 1