Uncovering the benefits of fluctuating thermal regimes on cold tolerance of drosophila flies by combined metabolomic and lipidomic approach

0461930 - BC 2017 RIV NL eng J - Journal Article
Colinet, H. - Renault, D. - Javal, M. - Berková, Petra - Šimek, Petr - Košťál, Vladimír
Uncovering the benefits of fluctuating thermal regimes on cold tolerance of drosophila flies by combined metabolomic and lipidomic approach.
Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. Roč. 1861, č. 11 (2016), s. 1736-1745. ISSN 1388-1981. E-ISSN 1879-2618
R&D Projects: GA ČR GA13-18509S
Institutional support: RVO:60077344
Keywords : cold stress * fluctuating thermal regimes * recovery
Subject RIV: ED - Physiology
Impact factor: 5.547, year: 2016
http://www.sciencedirect.com/science/article/pii/S1388198116302281

In this study, we have shown that Fluctuating temperature regimes (FTRs) strongly promoted cold tolerance of Drosophilamelanogaster adults. We then assessed whether this marked phenotypic shift was associated with detectable physiological changes, such as synthesis of cryoprotectants and/or membrane remodeling. To test these hypotheses, we conducted two different time-series Omics analyzes in adult flies submitted to CLTs vs. FTRs: metabolomics (GC/MS) and lipidomics (LC/ESI/MS) targeting membrane phospholipids. We observed increasing levels in several polyhydric alcohols, sugars and amino acids in flies under CLT. Lipidomics revealed an increased relative proportion of phosphatidylethanolamines and a shortening of fatty acyl chains in flies exposed to cold.

Permanent Link: http://hdl.handle.net/11104/0263669