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Automatic Image-Based Method for Quantitative Analysis of Photosynthetic Cell Cultures
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SYSNO ASEP 0460374 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Automatic Image-Based Method for Quantitative Analysis of Photosynthetic Cell Cultures Author(s) Vlachyňská, A. (CZ)
Červený, Jan (UEK-B) RID, ORCID, SAI
Cmiel, V. (CZ)
Tureček, T. (CZ)Source Title Hybrid Artificial Intelligent Systems: 11th International Conference, HAIS 2016, Seville, Spain, April 18-20, 2016, Proceedings. - Cham : Springer International Publishing, 2016 / Martínez-Álvarez F. ; Troncoso A. ; Quintián H. ; Corchado E. - ISSN 0302-9743 - ISBN 978-3-319-32033-5 Pages s. 402-103 Number of pages 12 s. Publication form Online - E Action International Conference on Hybrid Artificial Intelligent Systems /11./ Event date 18.04.2016 - 20.04.2016 VEvent location Seville Country ES - Spain Event type EUR Language eng - English Country CH - Switzerland Keywords automated cell counting ; image segmentation ; plant cell ; suspension cultures ; 3D reconstruction ; visualization Subject RIV EH - Ecology, Behaviour R&D Projects LO1415 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support RVO:67179843 - RVO:67179843 UT WOS 000389499600034 EID SCOPUS 84964040128 DOI 10.1007/978-3-319-32034-2_34 Annotation This work deals with an automatic quantitative analysis of photosynthetic cell cultures. It uses images captured by a confocal fluorescent microscope for automatic determination the number of cells in sample containing complex 3D structure of cell clusters. Experiments were performed on the confocal microscope Leica TCS SP8 X. The cell nuclei were stained by SYBR® Green fluorescent DNA binding marker. In the first step we used combination of adaptive thresholding to found out areas where nuclei were located. Proposed segmentation steps allowed reduction of noise and artefacts. Z-axis position was obtained as a location of peak from intensity profile. Finally model of scene can be created by emplacement of spheres with adequate diameter to found 3D coordinates. Number of cells per volumetric unit were determined in structurally different culture samples of cell cultures Chenopodium rubrum (Cr) and Solanum lycopersicum (To). The results were verified by manual counting Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2017
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