Treatment of alkali-injured cornea by cyclosporine A-loaded electrospun nanofibers - An alternative mode of therapy.

0460262 - ÚEM 2017 RIV GB eng J - Journal Article
Čejková, Jitka - Čejka, Čestmír - Trošan, Peter - Zajícová, Alena - Syková, Eva - Holáň, Vladimír
Treatment of alkali-injured cornea by cyclosporine A-loaded electrospun nanofibers - An alternative mode of therapy.
Experimental Eye Research. Roč. 147, may. (2016), s. 128-137. ISSN 0014-4835. E-ISSN 1096-0007
R&D Projects: GA ČR(CZ) GA14-12580S; GA MZd(CZ) NT14102; GA MŠMT(CZ) LO1309; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:68378041
Keywords : alkali injury * central corneal thickness * CsA-loaded nanofibers * immunohistochemistry
Subject RIV: FF - HEENT, Dentistry
Impact factor: 3.332, year: 2016

Introduction: Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic nanoparticles (PLL-coated gamma-Fe2O3) and studied their effect on proliferation and neuronal differentiation.

Materials and methods: We investigated the effect of these two contrast agents on neural precursor cell proliferation and differentiation capability. We further defined the intracellular localization and labeling efficiency and analyzed labeled cells by MR.

Results: Cell proliferation was not affected by PLL-coated gamma-Fe2O3 but was slowed down in cells labeled with CZF. Labeling efficiency, iron content and relaxation rates measured by MR were lower in cells labeled with CZF when compared to PLL-coated gamma-Fe2O3. Cytoplasmic localization of both types of nanoparticles was confirmed by transmission electron microscopy. Flow cytometry and immunocytochemical analysis of specific markers expressed during neuronal differentiation did not show any significant differences between unlabeled cells or cells labeled with both magnetic nanoparticles.

Conclusion: Our results show that cells labeled with PLL-coated gamma-Fe2O3 are suitable for MR detection, did not affect the differentiation potential of iPSC-NPs and are suitable for in vivo cell therapies in experimental models of central nervous system disorders.
Permanent Link: http://hdl.handle.net/11104/0260388