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Pure non-dioxin-like PCB congeners suppress induction of AhR-dependent endpoints in rat liver cells
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SYSNO ASEP 0456569 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Pure non-dioxin-like PCB congeners suppress induction of AhR-dependent endpoints in rat liver cells Author(s) Brenerová, P. (CZ)
Hamers, T. (NL)
Kamstra, J.H. (NL)
Vondráček, Jan (BFU-R) RID, ORCID
Střapacova, S. (CZ)
Andersson, P.L. (SE)
Machala, M. (CZ)Number of authors 7 Source Title Environmental Science and Pollution Research. - : Springer - ISSN 0944-1344
Roč. 23, č. 3 (2016), s. 2099-2107Number of pages 9 s. Publication form Print - P Language eng - English Country DE - Germany Keywords NDL-PCBs ; Aryl hydrocarbon receptor ; DR-CALUX (R) assay Subject RIV BO - Biophysics R&D Projects GBP503/12/G147 GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000368376800014 DOI 10.1007/s11356-015-4819-6 Annotation The relative potencies of non-ortho-substituted coplanar polychlorinated biphenyl (PCB) congeners to activate the aryl hydrocarbon receptor (AhR) and to cause the AhR-dependent toxic events are essential for their risk assessment. Since some studies suggested that abundant non-dioxin-like PCB congeners (NDL-PCBs) may alter the AhR activation by PCB mixtures and possibly cause non-additive effects, we evaluated potential suppressive effects of NDL-PCBs on AhR activation, using a series of 24 highly purified NDL-PCBs. We investigated their impact on the model AhR agonist-induced luciferase reporter gene expression in rat hepatoma cells and on induction of CYP1A1/1B1 mRNAs and deregulation of AhR-dependent cell proliferation in rat liver epithelial cells. PCBs 128, 138, and 170 significantly suppressed AhR activation (with IC50 values from 1.4 to 5.6 mu M), followed by PCBs 28, 47, 52, and 180; additionally, PCBs 122, 153, and 168 showed low but still significant potency to reduce luciferase activity. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2016
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