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A combined MR and CT study for precise quantitative analysis of the avian brain
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SYSNO ASEP 0454204 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A combined MR and CT study for precise quantitative analysis of the avian brain Author(s) Jirák, D. (CZ)
Janáček, Jiří (FGU-C) RID, ORCID
Kear, B. P. (SE)Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 5, Oct 30 (2015), s. 16002Number of pages 7 s. Language eng - English Country GB - United Kingdom Keywords avian brain ; magnetic resonance imaging ; computed tomography ; Fakir probe Subject RIV EA - Cell Biology R&D Projects GAP302/12/1207 GA ČR - Czech Science Foundation (CSF) Institutional support FGU-C - RVO:67985823 UT WOS 000363760700001 EID SCOPUS 84946105807 DOI 10.1038/srep16002 Annotation Brain size is widely used as a measure of behavioural complexity and sensory-locomotive capacity in avians but has largely relied upon laborious dissections, endoneurocranial tissue displacement, and physical measurement to derive comparative volumes. As an alternative, we present a new precise calculation method based upon coupled magnetic resonance (MR) imaging and computed tomography (CT). Our approach utilizes a novel interactive Fakir probe cross-referenced with an automated CT protocol to efficiently generate total volumes and surface areas of the brain tissue and endoneurocranial space, as well as the discrete cephalic compartments. We also complemented our procedures by using sodium polytungstate (SPT) as a contrast agent. This greatly enhanced CT applications but did not degrade MR quality and is therefore practical for virtual brain tissue reconstructions employing multiple imaging modalities. To demonstrate our technique, we visualized sex-based brain size differentiation in a sample set of Ring-necked pheasants (Phasianus colchicus). This revealed no significant variance in relative volume or surface areas of the primary brain regions. Rather, a trend towards isometric enlargement of the total brain and endoneurocranial space was evidenced in males versus females, thus advocating a non-differential sexually dimorphic pattern of brain size increase amongst these facultatively flying birds Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2016
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