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Raman tweezers in microfluidic systems for analysis and sorting of living cells
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SYSNO ASEP 0437742 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Raman tweezers in microfluidic systems for analysis and sorting of living cells Author(s) Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Ježek, Jan (UPT-D) RID, ORCID, SAI
Kaňka, Jan (UPT-D) RID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCIDNumber of authors 4 Source Title 19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE 9441). - Bellingham : SPIE, 2014 - ISSN 0277-786X - ISBN 9781628415568 Pages 944107:1-8 Number of pages 8 s. Publication form Print - P Action Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics /19./ Event date 08.09.2014-12.09.2014 VEvent location Jelenia Góra Country PL - Poland Event type EUR Language eng - English Country US - United States Keywords cells ; chemicals ; fluorescent markers ; optical analysis ; optical trappingo ; organisms ; Raman spectroscopy ; proteins Subject RIV BH - Optics, Masers, Lasers R&D Projects FR-TI1/433 GA MPO - Ministry of Industry and Trade (MPO) TA03010642 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 UT WOS 000349332600006 DOI 10.1117/12.2176047 Annotation We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment, dedicated to identification and sorting of biological objects, such as living cells of various unicellular organisms. Our main goal was to create a robust and universal platform for non-destructive and non-contact sorting of micro-objects based on their Raman spectral properties. This approach allowed us to collect spectra containing information about the chemical composition of the objects, such as the presence and composition of pigments, lipids, proteins, or nucleic acids, avoiding artificial chemical probes such as fluorescent markers. The non-destructive nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used a mixture of polystyrene micro-particles and algal cells to test and demonstrate the function of our analytical and sorting system. The devised system could find its use in many medical, biotechnological, and biological applications. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2015
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