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Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study
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SYSNO ASEP 0392660 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Polymorphism of human telomeric quadruplex structure controlled by DNA concentration: a Raman study Author(s) Palacký, J. (CZ)
Vorlíčková, Michaela (BFU-R) RID, ORCID
Kejnovská, Iva (BFU-R) RID, ORCID
Mojzeš, P. (CZ)Number of authors 4 Source Title Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 41, č. 2 (2013), s. 1005-1016Number of pages 12 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords INTRAMOLECULAR G-QUADRUPLEX ; K+ SOLUTION ; B-DNA Subject RIV BO - Biophysics R&D Projects GA202/09/0193 GA ČR - Czech Science Foundation (CSF) GAP205/12/0466 GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 CEZ AV0Z50040702 - BFU-R (2007-2013) UT WOS 000314121100034 DOI 10.1093/nar/gks1135 Annotation DNA concentration has been recently suggested to be the reason why different arrangements are revealed for K+-stabilized human telomere quadruplexes by experimental methods requiring DNA concentrations differing by orders of magnitude. As Raman spectroscopy can be applied to DNA samples ranging from those accessible by absorption and CD spectroscopies up to extremely concentrated solutions, gels and even crystals; it has been used here to clarify polymorphism of a core human telomeric sequence G(3)(TTAG(3))(3) in the presence of K+ and Na+ ions throughout wide range of DNA concentrations. We demonstrate that the K+-structure of G(3)(TTAG(3))(3) at low DNA concentration is close to the antiparallel fold of Na+-stabilized quadruplex. On the increase of G(3)(TTAG(3))(3) concentration, a gradual transition from antiparallel to intramolecular parallel arrangement was observed, but only for thermodynamically equilibrated K+-stabilized samples. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2014
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