Biotransformation of benzonitrile herbicides via the nitrile hydratase-amidase pathway in rhodococci

0387021 - MBÚ 2013 RIV DE eng J - Journal Article
Veselá, Alicja Barbara - Pelantová, Helena - Šulc, Miroslav - Macková, M. - Lovecká, P. - Thimová, P. - Pasquarelli, F. - Pičmanová, Martina - Pátek, Miroslav - Bhalla, T. C. - Martínková, Ludmila
Biotransformation of benzonitrile herbicides via the nitrile hydratase-amidase pathway in rhodococci.
Journal of Industrial Microbiology & Biotechnology. Roč. 39, č. 12 (2012), s. 1811-1819. ISSN 1367-5435. E-ISSN 1476-5535
R&D Projects: GA MŠMT OC09046; GA ČR(CZ) GAP504/11/0394; GA ČR GD305/09/H008
Keywords : Nitrile hydratase * Amidase * Benzonitrile herbicides
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.321, year: 2012

The aim of this work was to determine the ability of rhodococci to transform 3,5-dichloro-4-hydroxybenzonitrile (chloroxynil), 3,5-dibromo-4-hydroxybenzonitrile (bromoxynil), 3,5-diiodo-4-hydroxybenzonitrile (ioxynil) and 2,6-dichlorobenzonitrile (dichlobenil); to identify the products and determine their acute toxicities. Rhodococcus erythropolis A4 and Rhodococcus rhodochrous PA-34 converted benzonitrile herbicides into amides, but only the former strain was able to hydrolyze 2,6-dichlorobenzamide into 2,6-dichlorobenzoic acid, and produced also more of the carboxylic acids from the other herbicides compared to strain PA-34. Transformation of nitriles into amides decreased acute toxicities for chloroxynil and dichlobenil, but increased them for bromoxynil and ioxynil. The amides inhibited root growth in Lactuca sativa less than the nitriles but more than the acids. The conversion of the nitrile group may be the first step in the mineralization of benzonitrile herbicides but cannot be itself considered to be a detoxification
Permanent Link: http://hdl.handle.net/11104/0217221