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Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM)
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SYSNO ASEP 0381851 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM) Author(s) Strachotová, Dita (MBU-M)
Holoubek, A. (CZ)
Kučerová, Helena (MBU-M)
Benda, Aleš (UFCH-W) RID, ORCID
Humpolíčková, Jana (UFCH-W) RID
Váchová, Libuše (MBU-M) RID, ORCID
Palková, Z. (CZ)Source Title Biochimica Et Biophysica Acta-Biomembranes. - : Elsevier - ISSN 0005-2736
Roč. 1818, č. 9 (2012), s. 2126-2134Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords Ammonium exporters Ato1p ; Ato2p and Ato3p ; FLIM-photobleaching technique Subject RIV CE - Biochemistry R&D Projects GA204/08/0718 GA ČR - Czech Science Foundation (CSF) LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC531 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000306882600006 DOI 10.1016/j.bbamem.2012.05.005 Annotation Each of the three plasma membrane Ato proteins is involved in ammonium signalling and the development of yeast colonies. This suggests that although these proteins are homologous, they do not functionally substitute for each other, but may form a functional complex. Here, we present a detailed combined FRET, FLIM and photobleaching study, which enabled us to detect interactions between Ato proteins found in distinct compartments of yeast cells. We thus show that the proteins Ato1p and Ato2p interact and can form complexes when present in the plasma membrane. No interaction was detected between Ato1p and Ato3p or Ato2p and Ato3p. In addition, using specially prepared strains, we were able to detect an interaction between molecules of the same Ato protein, namely Ato1p-Ato1p and Ato3p-Ato3p, but not Ato2p-Ato2p. (C) 2012 Elsevier B.V. All rights reserved Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2013
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