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The Psb27 Assembly Factor Binds to the CP43 Complex of Photosystem II in the Cyanobacterium Synechocystis sp PCC 6803
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SYSNO ASEP 0378820 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Psb27 Assembly Factor Binds to the CP43 Complex of Photosystem II in the Cyanobacterium Synechocystis sp PCC 6803 Author(s) Komenda, Josef (MBU-M) RID, ORCID
Knoppová, Jana (MBU-M) RID
Kopečná, Jana (MBU-M) RID
Sobotka, Roman (MBU-M) RID, ORCID
Halada, Petr (MBU-M) RID, ORCID
Yu, J. (GB)
Nickelsen, J. (DE)
Boehm, M. (GB)
Nixon, P. J. (GB)Source Title Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 158, č. 1 (2012), s. 476-486Number of pages 11 s. Language eng - English Country US - United States Keywords MEMBRANE-PROTEIN COMPLEXES ; SP PCC-6803 ; D1 PROTEIN Subject RIV EE - Microbiology, Virology R&D Projects GAP501/11/0377 GA ČR - Czech Science Foundation (CSF) GAP501/10/1000 GA ČR - Czech Science Foundation (CSF) LC07017 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000298662500039 DOI 10.1104/pp.111.184184 Annotation We have investigated the location of the Psb27 protein and its role in photosystem (PS) II biogenesis in the cyanobacterium Synechocystis sp. PCC 6803. Native gel electrophoresis revealed that Psb27 was present mainly in monomeric PSII core complexes but also in smaller amounts in dimeric PSII core complexes, in large PSII supercomplexes, and in the unassembled protein fraction. We conclude from analysis of assembly mutants and isolated histidine-tagged PSII subcomplexes that Psb27 associates with the "unassembled" CP43 complex, as well as with larger complexes containing CP43, possibly in the vicinity of the large lumenal loop connecting transmembrane helices 5 and 6 of CP43. A functional role for Psb27 in the biogenesis of CP43 is supported by the decreased accumulation and enhanced fragmentation of unassembled CP43 after inactivation of the psb27 gene in a mutant lacking CP47 Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2013
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