Sperm surface proteins: their isolation, characterization and binding study with zona pellucida

0367654 - BTÚ 2012 AU eng A - Abstract
Zigo, Michal - Dorosh, Andriy - Jonáková, Věra - Postlerová, Pavla
Sperm surface proteins: their isolation, characterization and binding study with zona pellucida.
The book of abstracts of the World Congress on Reproductive Biology. Cairns: Society for Reproductive biology, 2011 - (Russell, D.; Okuda, K.; FitzHarris, G.). s. 134-134
[The Second Worl Congress on Reproductive Biology. 09.10.2011-12.10.2011, Cairns]
R&D Projects: GA ČR(CZ) GA303/09/1285; GA MŠMT(CZ) 1M06011; GA MZd(CZ) NS10009-4/2008
Institutional research plan: CEZ:AV0Z50520701
Keywords : sperm surface proteins * zona pellucida * glycoproteins
Subject RIV: CE - Biochemistry

Binding of sperms to the oocyte is mediated by complementary molecules on the surface of both gametes and involves the interaction of sperm protein receptors with the zona pellucida (ZP) saccharide structures. Sperm surface proteins were isolated from ejaculated and capacitated boar sperms and characterized 1D, 2D protein profiles, glycoprotein staining and substrate zymographic methods for enzymatic activities. Interactions between sperm surface proteins of both ejaculated and capacitated sperms with ZP glycoproteins were studied. Panel of monoclonal antibodies (MoAbs) against sperm surface proteins was prepared; antibodies were screened for protein detection with ZP-binding activity. Results have shown the quantitative and qualitative differences in 1D and 2D protein, and glycoprotein profiles between ejaculated and capacitated sperms. ZP-binding assay proved more interactions in case of capacitated sperms. Some MoAbs were staining regions responsible for ZP-binding.
Permanent Link: http://hdl.handle.net/11104/0202247