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Distribution and development of the postnatal murine V delta 1 T-cell receptor repertoire
- 1.0352860 - MBÚ 2011 RIV GB eng J - Journal Article
Holtmeier, W. - Gille, J. - Zeuzem, S. - Šinkora, Marek
Distribution and development of the postnatal murine V delta 1 T-cell receptor repertoire.
Immunology. Roč. 131, č. 2 (2010), s. 192-201. ISSN 0019-2805. E-ISSN 1365-2567
R&D Projects: GA ČR GA524/07/0087
Institutional research plan: CEZ:AV0Z50200510
Keywords : gene rearrangement * repertoire development * rodent
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.302, year: 2010
Murine gamma/delta T cells express canonical V gamma 5V delta 1 chains in the epidermis and V gamma 6V delta 1 chains at reproductive sites. Both subsets carry an identical V delta 1-D delta 2-J delta 2 chain which completely lacks junctional diversity. These cells are thought to monitor tissue integrity via recognition of stress-induced self antigens. In this study, we showed by reverse transcription-polymerase chain reaction (RT-PCR), complementarity determining region 3 (CDR3) spectratyping and sequencing of the junctional regions of V delta 1 chains from C57BL/6 mice (aged 1 day to 14 months) that the canonical V delta 1-D delta 2-J delta 2 chain is also consistently present at other sites such as the thymus, gut, lung, liver, spleen and peripheral blood
Permanent Link: http://hdl.handle.net/11104/0192264
Number of the records: 1