Electrochemical enzyme-linked immunoassay in a DNA hybridization sensor

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SYSNO ASEP	0126749
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Electrochemical enzyme-linked immunoassay in a DNA hybridization sensor
Author(s)	Paleček, Emil (BFU-R)_{RID, ORCID} Kizek, René (BFU-R) Havran, Luděk (BFU-R)_{RID, ORCID} Billová, Sabina (BFU-R) Fojta, Miroslav (BFU-R)_{RID, ORCID}
Source Title	Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670 Roč. 469, č. 1 (2002), s. 73-83
Number of pages	11 s.
Language	eng - English
Country	NL - Netherlands
Keywords	electrochemical sensor for DNA hybridization ; enzyme-linked immunoassay of target DNA ; magnetic beads with attached DNA probe
Subject RIV	BO - Biophysics
R&D Projects	GV204/97/K084 GA ČR - Czech Science Foundation (CSF)
	GA204/00/D049 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z5004920 - BFU-R
Annotation	In most of the currently developed electrochemical DNA hybridization sensors short single-stranded probe DNA is immobilized on an electrode and both the hybridization and detection steps are carried out on the electrode surface. Here we use a new technology in which DNA hybridization is performed on commercially available magnetic beads and detection on solid electrodes. Paramagnetic Dynabeads Oligo(dT)25 (DBT) with covalently bound (dT)25 probe are used for the hybridization with target DNA containing adenine stretches.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2003

Number of the records: 1