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Factors influencing resistance of UV-irradiated DNA to the restriction endonuclease cleavage
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SYSNO ASEP 0104655 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Factors influencing resistance of UV-irradiated DNA to the restriction endonuclease cleavage Title Faktory ovlivňující odolnost DNA ozářené ultrafialovým světlem vůči štěpení restrikčními endonukleasami Author(s) Kejnovský, Eduard (BFU-R) RID, ORCID
Nejedlý, Karel (BFU-R) RID
Kypr, Jaroslav (BFU-R) RIDSource Title International Journal of Biological Macromolecules. - : Elsevier - ISSN 0141-8130
Roč. 34, č. 3 (2004), s. 213-222Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords DNA ; structure ; damage Subject RIV BO - Biophysics R&D Projects IAA5004802 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IAA1004201 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IAA1004301 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z5004920 - BFU-R Annotation DNA molecules of pUC19, pBR322 and PhiX174 were irradiated by various doses of UV light and the irradiated molecules were cleaved by about two dozen type II restrictases. The irradiation generally blocked the cleavage in a dose-dependent way. In accordance with previous studies, the (A + T)-richness and the (PyPy) dimer content of the restriction site belongs among the factors that on average, cause an increase in the resistance of UV damaged DNA to the restrictase cleavage. However, we observed strong effects of UV irradiation even with (G + C)-rich and (PyPy)-poor sites. In addition, sequences flanking the restriction site influenced the protection in some cases (e.g. HindIII), but not in others (e.g. SalI), whereas neoschizomer couples SmaI and AvaI, or SacI and Ecl136II, cleaved the UV-irradiated DNA similarly. Hence the intrastrand thymine dimers located in the recognition site are not the only photoproduct blocking the restrictases. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2005
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