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Improvement in volume estimation from confocal sections after image deconvolution
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SYSNO ASEP 0103084 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Improvement in volume estimation from confocal sections after image deconvolution Title Zpřesnění odhadu objemu z konfokálních řezů po aplikaci dekonvoluce obrazu Author(s) Difato, Francesco (FGU-C)
Mazzone, F. (IT)
Scaglione, S. (IT)
Fato, M. (IT)
Beltrame, F. (IT)
Kubínová, Lucie (FGU-C) RID, ORCID
Janáček, Jiří (FGU-C) RID, ORCID
Ramoino, P. (IT)
Vicidomini, G. (IT)
Diaspro, A. (IT)Source Title Microscopy Research and Technique - ISSN 1059-910X
Roč. 64, č. 2 (2004), s. 151-155Number of pages 5 s. Language eng - English Country US - United States Keywords confocal microscopy ; image deconvolution ; point spread function Subject RIV EA - Cell Biology CEZ AV0Z5011922 - FGU-C Annotation The confocal fluorescence microscope is considered as a true volume imager but intrinsic imaging properties of the optical scheme yield a corrupted image that can hamper quantitative analysis of successive image planes. By a post-image collection restoration we obtained an estimate of the true object, utilizing the Point Spread Function (PSF).The PSF was measured and predicted to have a mathematical and physical model of the image-formation process. We used the regularized Iterative Constrained Tykhonov Miller restoration algorithm for solving the inverse problem. In order to compare the effective improvement in the quantitative image information analysis, we measured the volume of reference objects before and after image restoration, using the isotropic fakir stereological method Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2005
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