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Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions
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SYSNO ASEP 0043210 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Lipid diffusion in giant unilamellar vesicles is more than 2 times faster than in supported phospholipid bilayers under identical conditions Title Difúze lipidů v obřích unilamelárních vesiklech je více než dvakrát rychlejší než difúze v podporovaných fosfolipidových membránách za totožných podmínek Author(s) Przybylo, Magdalena (UFCH-W)
Sýkora, Jan (UFCH-W) RID
Humpolíčková, Jana (UFCH-W) RID
Benda, Aleš (UFCH-W) RID, ORCID
Zań, Anna (UFCH-W)
Hof, Martin (UFCH-W) RID, ORCIDSource Title Langmuir. - : American Chemical Society - ISSN 0743-7463
Roč. 22, č. 22 (2006), s. 9096-9099Number of pages 4 s. Language eng - English Country US - United States Keywords fluorescence-correlation spectroscopy ; model membranes ; molecular friction Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA203/05/2308 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40400503 - UFCH-W (2005-2011) Annotation The lateral diffusion coefficients of a BODIPY tail-labeled lipid in two model systems, namely, free-standing giant unilamellar vesicles (GUVs) and supported phospholipid bilayers (SPBs), were determined by fluorescence correlation spectroscopy (FCS) using the Z-scan approach. For the first time, the performed measurements on 1,2-dioleoyl-sn-glycero-3-phosphocholine ( DOPC) bilayers maintain exactly the same experimental conditions for both systems, which allows for a quantitative comparison of lipid diffusion in these two commonly used model membranes. The results obtained revealed that the lipid mobility in free-standing bilayers (D = 7.8 +/- 0.8 mu m(2) s(-1)) is significantly higher than in the bilayer created on the solid support (mica) (D = 3.1 +/- 0.3 mu m(2) s(-1)). Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2007
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